Multi-disciplinary investigations are being undertaken in the ICNT on the molecular basis of communication in the nervous system, especially identifying and structurally characterising the proteins responsible for the fundamental process of quantal release of transmitters, and its indirect regulation by voltage-sensitive K+ channels.

Our basic research on the selective and potent inhibition of transmitter release by botulinum neurotoxins has underpinned their successful and worldwide clinical use in treating human dystonias, spasticity and other movement disorders, as well as autonomic neuronal abnormalities of secretory glands (e.g. hyper-hydrosis, -salivation and lacrimation etc.), over-active bladder (e.g. in spina bifida, spinal cord injuries and multiple sclerosis) and gastrointestinal tract (e.g. pyloric sphincter). Current efforts are devoted to developing second generation homologues and tailoring their functional properties for novel therapies. Also, information is being sought on the molecular triggers for nerve sprouting induced by blockade of acetylcholine release due to cleavage of SNAP-25 by botulinum toxin A (1), and the eventual retraction of these neurites, in relation to the extraordinary duration (many months) of therapeutic benefit it offers (2). Likewise, individual steps of exocytosis are being deciphered and the function domains identified by overcoming the toxin’s inhibition of exocytosis (3) by expressing recombinant variants of SNAP-25.

Several years of research on neuronal K+ channels have been devoted to elucidating their oligomeric/primary structures (4, 5) and deciphering the genetic defects in these proteins in certain human channelopathies. Such molecular definition of heteromeric subtypes in normal and diseased states (6), coupled with their reconstruction by recombinant means (7), are providing authentic targets for drug refinement/development. This has been achieved by tandem linkage of the genes encoding different constituents of neuronal voltage-activated K+ channels (KvI). Subsequent expression of these proteins on the surface of mammalian cells generates channel oligomers with pre-defined combinations and stoichiometres of subunits which mimic the subtypes found in human brain. Similar protein engineering can produce a unique K+ channel that appears in demyelinated axons from patients with multiple sclerosis, and those containing identified mutations (e.g. with inherited channelopathies in children). By these means, existing K+ channel drugs are to be tested for abilities to act discriminatively on one of the normal or abnormal K+ channel subtypes with the eventual goal of being able to modify neuronal excitability.

1. de Paiva, A., Meunier, F.A., Molgo, J., Aoki, R. and Dolly, J. O. 'Functional repair of motor endplates after botulinum toxin-A poisoning: Bi-phasic switch of synaptic activity between nerve sprouts and their parent terminals' Proc. Natl. Acad. Sci. (USA) 96, (1999), 3200-3205.
2. Meunier, F. A., Lisk, G., Sesardic, D. and Dolly, J. O. 'Dynamics of motor nerve terminal remodeling unveiled using SNARE-cleaving botulinum toxins: the extent and duration are dictated by the sites of SNAP-25 truncation' Molecular and Cellular Neuroscience 22, (2003).
3. O’Sullivan, G.A., Mohammed, N., Foran, P.G., Lawrence, G.W. and Dolly, J.O. 'Rescue of exocytosis in botulinum toxin A-poisoned chromaffin cells by expression of cleavage-resistant SNAP-25: identification of the minimal essential C-terminal residues' J. Biol. Chem. 274, (1999), 36897-36904.
4. Shamotienko, O., Akhtar, S., Sidera, C., Meunier, F.A., Ink, B., Weir, M. and Dolly, J.O. 'Recreation of neuronal Kv1 channel oligomers by expression in mammalian cells using Semliki Forest virus' Biochemistry 38, (1999), 16766-16776.
5. Orlova, E. V., Papakosta, M., Booy, F. P., van Heel, M. and Dolly, J. O. 'Voltage-gated K+ channel from mammalian brain: (?)4 (?)4 complex J. Mol. Biol. 326 (2003) 1005-1012.
6. Manganas, L. N., Akhtar, S., Antonucci, D. E., Campomanes, C. R., Dolly, J. O. and Trimmer, J. S. 'Episodic ataxia type-1 mutations in the Kv1.1 potassium channel display distinct folding and intracellular trafficking properties' J. Biol. Chem. 276, (2001), 49427-49434.
7. Akhtar, S., Shamotienko, O., Papakosta, M., Ali, F. and Dolly, J.O. ‘Characteristics of brain Kv1 channels tailored to mimic native counterparts by tandem-linkage of a subunits: implications for K+ channelopathies’ J. Biol. Chem. 277, (2002), 16376-16382.

TEAM MEMBERS' RECENT PUBLICATIONS (1999-2004) BY DATE:

Dr MacDara Bodeker:

2003:

• Oster, S., Bodeker, M., He, F., and Sretavan, D. W. (2003). Invariant Sema5A inhibition serves an ensheathing function during optic nerve development. Development 130, 775-784.
• Ellison, A., Yang, L., Bodeker, M., Matthews, Pakpour, N., and Margolis, T. P. (2003). Functional Cloning of Neuronal Genes that Inhibit HSV IE3 Promoter Activity. Manuscript in preparation.

2004:

• Bodeker, M., Matthews, T. P., Warren, J., and Margolis, T. P. (2004). A Novel KLF Family Splice Variant Consisting of Only a KRAB-A Box Is Expressed in the Murine Trigeminal Ganglia. Manuscript submitted.
• Yoon, M., Bodeker, M., Imai, Y., and Margolis, T. P. (2004). The Role of YY1 at the HSV-1 Immediate Early Gene Promoter. Manuscript in preparation.

Dr. Oleg Chamotienko:

1999:

• Shamotienko, O., Akhtar, S., Sidera, C., Meunier, F.A., Ink, B., Weir, M. and Dolly, J.O. (1999). Recreation of Neuronal Kv1 Channel Oligomers by Expression in Mammalian Cells Using Semliki Forest Virus. Biochem 38 (51), 16766-16776.

2000:

• Poopalasundaram, S., Knott, C.H., Shamotienko, O., Foran, P., Dolly, J.O., Ghiani, C.A. Gallo, V., and Wilkin, G.P. (2000). Glial Heterogenity in Expression of the Inwardly Rectifying K+ Channel, Kir 4.1, in Adult Rat CNS. Glia 30, 362-372.

2002:

• Akhtar, S, Shamotienko, O., and Dolly, J.O. (2002). Characteristics of Brain Kv1 channels Tailored to Mimic Native Counterparts by Tandem Linkage of ?-Subunits. J Biol Chem 227 (19), 16376-16382.
• Akhtar, S., Shamotienko, O., Papakosta, M., Ali, F., and Dolly, J.O. (2002). Characteristics of Brain Kv1 Channels Tailored to Mimic Native Counterparts by Tandem Linkage of Subunits. Implications for K+ channelopathies. J Biol Chem 277, 16376 - 16382.

Dr. Gary Lawrence:

1999:

• O'Sullivan, G. A., Mohammed, N., Foran, P. G., Lawrence, G. W., and Dolly, J.O. (1999). Rescue of exocytosis in botulinum toxin A-poisoned chromaffin cells by expression of cleavage-resistant SNAP-25. Identification of the minimal essential C-terminal residues. J Biol Chem 274, 36897-904.

2000:

• Meunier, F. A., Mattei, C., Chameau, P., Lawrence, G., Colasante, C., Kreger, A. S., Dolly, J. O., and Molgo, J. (2000). Trachynilysin mediates SNARE-dependent release of catecholamines from chromaffin cells via external and stored Ca2+. J Cell Sci 113, 1119-25.

2001:

• Li, Y.*, Foran, P.*, Lawrence, G.*, Mohammed, N., Chan-Kwo-Chion, C. K., Lisk, G., Aoki, R., and Dolly, O. (2001). Recombinant forms of tetanus toxin engineered for examining and exploiting neuronal trafficking pathways. J Biol Chem 276, 31394-401. (*Joint primary authorship)

2002:

• Lawrence, G. W. and Dolly, J. O. (2002). Ca2+-induced changes in SNAREs and synaptotagmin I correlate with triggered exocytosis from chromaffin cells: insights gleaned into the signal transduction using trypsin and botulinum toxins. J Cell Sci 115, 2791-800.
• Lawrence, G. W. and Dolly, J. O. (2002). Multiple forms of SNARE complexes in exocytosis from chromaffin cells: effects of Ca2+, MgATP and botulinum toxin type A. J Cell Sci, 115, 667-73.
• Lawrence, G. W., Foran, P., and Dolly, J. O. (2002). Insights into a basis for incomplete inhibition by botulinum toxin A of Ca2+-evoked exocytosis from permeabilised chromaffin cells. Toxicology 181-182, 249-253.

2003:

• Foran, P. G., Mohammed, N., Lisk, G. O., Nagwaney, S., Lawrence, G. W., Johnson, E., Smith, L., Aoki, K. R., and Dolly, J. O. (2003). Evaluation of the therapeutic usefulness of botulinum neurotoxin B, C1, E, and F compared with the long lasting type A. Basis for distinct durations of inhibition of exocytosis in central neurons. J Biol Chem 278, 1363-71.

Ms Jianghui Meng:

1999:

• Meng, J. and Chen, W (1999). Using Xanthine Oxidase to improve the productivity of ribavirin (RBV). J Wuhan Univ (Nat. Sci. Ed.) 45 (6), 841-844.
• Meng, J. and Chen, W (1999). A Survey of Study on Bacteria Xanthine Dehydrogenase. Amino Acid & Biotic Resources 21 (3), 52-55.
• Meng, J. and Chen, W RBV Synthesised by Two Strains of Bacteria. Chinese Invention Patent. Application No. 98121662.5

Dr Arvind Raghunath:

2001:

• Raghunath A., Coppell A., Heidbreder C., Wagner G., Albrecht U. and Dreyer J.-L..Identification of Cocaine-regulated Gene Transcripts and Modulation of Locomotor Behaviour by modifications in their Expression Levels Using a Lentiviral System. Society for Neuroscience, San Diego, California, November 10-15th, 2001.

Dr Astrid Sasse:

1999:

• Sasse, A., Kiec-Kononowicz, K., Stark, H., Motyl, M., Reidemeister, S., Ganellin, C. R., Ligneau, X., Schwartz, J.-C. and Schunack, W. (1999). Development of Chiral N-Alkylcarbamates as New Leads for Potent and Selective H3-Receptor Antagonists: Synthesis, Capillary Electrophoresis, and in Vitro and Oral in Vivo Activity. J Med Chem 42, 593-600.
• Sasse, A., Stark, H., Reidemeister, S., Hüls, A., Elz, S., Ligneau, X., Ganellin, C. R., Schwartz, J.-C. and Schunack, W. (1999). Novel Partial Agonists for the Histamine H3 Receptor with High in Vitro and in Vivo Activity. J Med Chem 42, 4269-4274.

2000:

• Sasse, A., Stark, H., Ligneau, X., Elz, S., Reidemeister, S., Ganellin, C.R., Schwartz, J.-C. and Schunack, W. (2000). (Partial) Agonist/Antagonist Properties of Novel Diarylalkyl Carbamates on Histamine H3 Receptors. Bioorg Med Chem 8, 1139-1149.
• Kiec-Kononowicz, K., Wiecek, M., Sasse, A., Ligneau, X., Elz, S., Ganellin, C. R., Schwartz, J.-C., Stark, H. and Schunack, W. (2000). Importance of the Lipophilic Group in Carbamates having Histamine H3-Receptor Antagonist Activity. Pharmazie 55, 349-355.
• Sasse, A., Sadek, B., Ligneau, X., Elz, S., Pertz, H., Luger, P., Ganellin, C. R., Arrang, J.-M., Schwartz, J.-C., Schunack, W. and Stark, H. (2000). New Histamine H3-Receptor Ligands of the Proxifan-Series: Imoproxifan and Other Selective Antagonists with High Oral in Vivo Potency. J Med Chem 43, 3335-3343.

2001:

• Sasse, A., Schunack, W. and Stark, H. (2001). Separation of Chiral 4-Substituted Imidazole Derivatives by Cyclodextrin-Modified Capillary Electrophoresis. Biomed Chromatogr 15, 25-30.
• Morisset, S., Sasse, A., Gbahou, F., Héron, A., Ligneau, X., Tarvidel-Lacombe, J., Schwartz, J.-C. and Arrang, J.-M. (2001). The Rat H3 Receptor: Gene Organization and Multiple Isoforms. Biochem Biophys Res Commun 280, 75-80.
• Nickel, T., Bauer, U., Schlicker, E., Kathmann, M., Göthert, M., Sasse, A., Stark, H. and Schunack, W. (2001). Novel Histamine H3-Receptor Antagonists and Partial Agonists with a Non-Aminergic Structure. Br J Pharmacol 132, 1665-1672.
• Morini, G., Grandi, D., Sasse, A., Stark, H. and Schunack, W. (2001). Gastroprotective Activity of the Novel Histamine H3-Receptor Agonist FUB 407. Inflamm Res 50 (Suppl. 2), S116-S117.
• Sasse, A., Ligneau, X., Sadek, B., Elz, S., Pertz, H.H., Ganellin, C. R., Arrang, J.-M., Schwartz, J.-C., Schunack, W. and Stark, H. (2001). Benzophenone Derivatives and Related Compounds as Potent Histamine H3-Receptor Antagonists and Potential PET/SPECT Ligands. Arch Pharm Pharm Med Chem 334, 45-52.

2002:

• Wieçek, M., Kiec-Kononowicz, K., Sasse, A., Ligneau, X., Schwartz, J.-C., Schunack, W. and Stark, H. (2002). Impact on the Pharmacological Properties of Histamine H3-Receptor Antagonists of the Cycloalkyl Carbamate Class. Inflamm Res 51 (Suppl. 1), S71-S72.
• Schwartz, J.-C., Morisset, S., Rouleau, A., Tarvidel-Lacombe, J., Gbahou, F., Ligneau, X., Héron, A., Sasse, A., Stark, H., Schunack, W., Ganellin, C. R. and Arrang, J.-M. (2002). Application of Genomics to Drug Design: The Example of the Histamine H3 Receptor. Eur Neuropsychopharmacol 11, 441-448.
• Sasse, A., Ligneau, X., Rouleau, A., Elz, S., Ganellin, C. R., Arrang, J.-M., Schwartz, J.-C., Schunack, W. and Stark, H. (2002). Influence of Bulky Substituents on Histamine H3 Receptor Agonist/Antagonist Properties. J Med Chem 45, 4000-4010.

2004:

• Sasse, A., Morisset, S., Krebs, M.-O., Bourdel, M.-C., Héron, A., Schwartz, J.-C. and Arrang, J.-M. (2004) Polymorphisms of the Histamine H4-Receptor Gene: Is there an Association to Schizophrenia? (Manuscript in preparation).
• Tezval, H., Jahn, O., Todorovic, C., Sasse, A., Eckart, K. and Spiess, J. (2004) Cortagine, a New CRF1 Receptor-Specific Agonist, Displays Anxiogenic and Anti-depressive Properties in the Mouse Model. Proc. Natl. Acad. Sci. USA 101, 9468-9473.

Dr Jiafu Wang:

2000:

• Huang, Q.H., Zhang, C.Y., Wang, J.F., Fu, L.Z., Wang, N., Zhu, Y., Huai, J.S., Zhang, P.W., Yu, J.S. and Xu, H. (2000). Construction of a cDNA library, nucleotide sequence and analysis of entire genome of classical swine fever virus strain Shimen. Chinese Science Bulleting 45 (4), 367-370.
• Wang, J.F., Zhang, C.Y., Ding, J.H., Zhou, R., When, S.J., and Huang, Z.H. (2000). Cloning & sequencing of gD gene of pseudorabies virus of Hubeu Strain. Chinese Journal of Virology 16 (1), 65-69.
• Wang, J.F., Zhang, C.Y., Fu, L.Z., Huang, Q.H. and Zhang P.W. (2000). Sequence analysis of NS2-3 gene of hog cholera virus Lapinized Chinese Strain. Chinese Journal of Virology 16 (2), 150-154.
• Wang, J.F., Zhang, C.Y., Wang, N., Fu, L.Z., and Huang, Q.H. (2000). Cloning and sequence analysis of E0 glycoprotein gene of hog cholera virus lapinized Chinese strain and virulent Shimen strain. Acta Microbiologica Sinica 40 (91), 32-37.

2001:

• Wu, H.X., Wang, J.F., Zhang, C.Y., Fu, L.Z., Pan, Z.S., Wang, N., Zhang, P.W., and Zhao W.G. (2001). Attenuated lapinized Chinese strain of classical swine fever virus: Complete nucleotide sequence and character of 3'-noncoding region. Virus Genes 23 (1), 69-76.

Dr Tom Zurawski:

1999:

• Zurawski T.H., Mair G.R., Maule A.G., Gelnar M., Koubková B., and Halton D.W., (1999). Confocal scanning laser microscopy of neuronal pathways and muscle systems of Eudiplozoon nipponicum (Monogenea: Diplozoidae), parasite of gill of carp (Cyprinus carpio). Helminthologia 36, Suppl. 5.

2000:

• Gelnar M., Hodová I., Koubková B., Šimková A., and Zurawski T.H., (2000). Microhabitat specificity of Eudiplozoon nipponicum (Diplozoidea, Monogenea) in relation to ontogenetic changes of the parasite tegumentary structures. Acta Parasitologica 45 (3), 261.
• Zurawski T.H., Mair G., Mousley A., Brennan G., Maule A., Gelnar M. and Halton D.W., (2000). Immunomicroscopical studies of the neuromusculature of the monogenean parasite, Eudiplozoon nipponicum. Acta Parasitologica 45 (3), 166.

2001:

• Zurawski, T.H, Mousley, A., Mair G., Brennan G., Maule A., Gelnar M. and Halton D.W., (2001). Immunomicroscopical observations on the nervous system of adult Eudiplozoon nipponicum (Monogenea: Diplozoidae). International Journal for Parasitology 31, 783-792.

2003:

• Zurawski T.H., Mair, G.R., Maule, A.G., Gelner, M., and Halton, D.W. (2003). Microscopic evaluation of neural connectivity between paired stages of Eudiplozoon nipponicum (Monogea: Diplozoidae). Journal of Parasitology 98, 198-200.
• Zurawski, T.H, Mousley, A., Maule, A.G., Gelner, M., and Halton, D.W. (2003). Cytochemical studies of the neuromuscular systems of the diporpa and juvenile stages Eudiplozoon nipponicum (Monogenea: Diplozoidae). Parasitology 126, 349-57.

2004:

• Horgan C.P., Walsh M., Zurawski T.H., and McCaffrey M.W., (2004). Rab11-FIP3 localises to a Rab11-positive pericentrosomal compartment during interphase and to the cleavage furrow during cytokinesis. Biochem. Biophys Res Commum 319 (1), 83-94.

Prof. Oliver Dolly

1999:

• Akhtar, S., McIntosh, P., Bryan-Sisneros, A., Barratt, L., Robertson, B., and Dolly, J. O. (1999). A functional spliced-variant of beta 2 subunit of Kv1 channels in C6 glioma cells and reactive astrocytes from rat lesioned cerebellum. Biochemistry 38, 16984-16992.
• de Paiva, A., Meunier, F. A., Molgo, J., Aoki, K. R., and Dolly, J. O. (1999). Functional repair of motor endplates after botulinum neurotoxin type A poisoning: biphasic switch of synaptic activity between nerve sprouts and their parent terminals. Proc Natl Acad Sci U S A 96, 3200-3205.
• Foran, P. G., Fletcher, L. M., Oatey, P. B., Mohammed, N., Dolly, J. O., and Tavare, J. M. (1999). Protein kinase B stimulates the translocation of GLUT4 but not GLUT1 or transferrin receptors in 3T3-L1 adipocytes by a pathway involving SNAP-23, synaptobrevin-2, and/or cellubrevin. J Biol Chem 274, 28087-28095.
• Li, Y., Aoki, R., and Dolly, J. O. (1999). Expression and characterisation of the heavy chain of tetanus toxin: reconstitution of the fully-recombinant dichain protein in active form. J Biochem (Tokyo) 125, 1200-1208.
• Rahman, M. A., Ashton, A. C., Meunier, F. A., Davletov, B. A., Dolly, J. O., and Ushkaryov, Y. A. (1999). Norepinephrine exocytosis stimulated by alpha-latrotoxin requires both external and stored Ca2+ and is mediated by latrophilin, G proteins and phospholipase C. Philos Trans R Soc Lond B Biol Sci 354, 379-386.
• Shamotienko, O., Akhtar, S., Sidera, C., Meunier, F. A., Ink, B., Weir, M., and Dolly, J. O. (1999). Recreation of neuronal Kv1 channel oligomers by expression in mammalian cells using Semliki Forest virus. Biochemistry 38, 16766-16776.
• Wang, F. C., Bell, N., Reid, P., Smith, L. A., McIntosh, P., Robertson, B., and Dolly, J. O. (1999). Identification of residues in dendrotoxin K responsible for its discrimination between neuronal K+ channels containing Kv1.1 and 1.2 alpha subunits. Eur J Biochem 263, 222-229.
• Coleman, S. K., Newcombe, J., Pryke, J., and Dolly, J. O. (1999). Subunit composition of Kv1 channels in human CNS. J Neurochem 73, 849-858.
• Wang, F. C., Parcej, D. N., and Dolly, J. O. (1999). alpha subunit compositions of Kv1.1-containing K+ channel subtypes fractionated from rat brain using dendrotoxins. Eur J Biochem 263, 230-237.

2000:

• Ashton, A. C., and Dolly, J. O. (2000). A late phase of exocytosis from synaptosomes induced by elevated [Ca2+]i is not blocked by Clostridial neurotoxins. J Neurochem 74, 1979-1988.
• Meunier, F. A., Mattei, C., Chameau, P., Lawrence, G., Colasante, C., Kreger, A. S., Dolly, J. O., and Molgo, J. (2000). Trachynilysin mediates SNARE-dependent release of catecholamines from chromaffin cells via external and stored Ca2+. J Cell Sci 113 ( Pt 7), 1119-1125.
• Poopalasundaram, S., Knott, C., Shamotienko, O. G., Foran, P. G., Dolly, J. O., Ghiani, C. A., Gallo, V., and Wilkin, G. P. (2000). Glial heterogeneity in expression of the inwardly rectifying K(+) channel, Kir4.1, in adult rat CNS. Glia 30, 362-372.
• Volynski, K. E., Meunier, F. A., Lelianova, V. G., Dudina, E. E., Volkova, T. M., Rahman, M. A., Manser, C., Grishin, E. V., Dolly, J. O., Ashley, R. H., and Ushkaryov, Y. A. (2000). Latrophilin, neurexin, and their signaling-deficient mutants facilitate alpha -latrotoxin insertion into membranes but are not involved in pore formation. J Biol Chem 275, 41175-41183.

2001:

• Manganas, L. N., Akhtar, S., Antonucci, D. E., Campomanes, C. R., Dolly, J. O., and Trimmer, J. S. (2001). Episodic ataxia type-1 mutations in the Kv1.1 potassium channel display distinct folding and intracellular trafficking properties. J Biol Chem 276, 49427-49434.

2002:

• Akhtar, S., Shamotienko, O., Papakosta, M., Ali, F., and Dolly, J. O. (2002). Characteristics of brain Kv1 channels tailored to mimic native counterparts by tandem linkage of alpha subunits: implications for K+ channelopathies. J Biol Chem 277, 16376-16382.
• Lawrence, G. W., and Dolly, J. O. (2002). Ca2+-induced changes in SNAREs and synaptotagmin I correlate with triggered exocytosis from chromaffin cells: insights gleaned into the signal transduction using trypsin and botulinum toxins. J Cell Sci 115, 2791-2800.
• Lawrence, G. W., and Dolly, J. O. (2002). Multiple forms of SNARE complexes in exocytosis from chromaffin cells: effects of Ca(2+), MgATP and botulinum toxin type A. J Cell Sci 115, 667-673.

2003:

• Foran, P. G., Mohammed, N., Lisk, G. O., Nagwaney, S., Lawrence, G. W., Johnson, E., Smith, L., Aoki, K. R., and Dolly, J. O. (2003). Evaluation of the therapeutic usefulness of botulinum neurotoxin B, C1, E, and F compared with the long lasting type A. Basis for distinct durations of inhibition of exocytosis in central neurons. J Biol Chem 278, 1363-1371.
• Fraser, S. P., Grimes, J. A., Diss, J. K., Stewart, D., Dolly, J. O., and Djamgoz, M. B. (2003). Predominant expression of Kv1.3 voltage-gated K+ channel subunit in rat prostate cancer cell lines: electrophysiological, pharmacological and molecular characterisation. Pflugers Arch 446, 559-571.
• Meunier, F. A., Lisk, G., Sesardic, D., and Dolly, J. O. (2003). Dynamics of motor nerve terminal remodeling unveiled using SNARE-cleaving botulinum toxins: the extent and duration are dictated by the sites of SNAP-25 truncation. Mol Cell Neurosci 22, 454-466.
• Orlova, E. V., Papakosta, M., Booy, F. P., van Heel, M., and Dolly, J. O. (2003). Voltage-gated K+ channel from mammalian brain: 3D structure at 18A of the complete (alpha)4(beta)4 complex. J Mol Biol 326, 1005-1012.
• Tang-Liu, D. D., Aoki, K. R., Dolly, J. O., de Paiva, A., Houchen, T. L., Chasseaud, L. F., and Webber, C. (2003). Intramuscular injection of 125I-botulinum neurotoxin-complex versus 125I-botulinum-free neurotoxin: time course of tissue distribution. Toxicon 42, 461-469.

2004:

• Bagetta, G., Palma, E., Piccirilli, S., Del Duca, C., Morrone, A. L., Nappi, G., Corasaniti, M. T., and Dolly, J. O. (2004). Involvement of a glutamergic mechanism in gamma-dendrotoxin-induced hippocampal neuronal cell loss in the rat. Basic Clin Pharmacol Toxicol 94, 132-138.
• Shimamura, T., Shamotienko, O., Akhtar, S., Dolly, J. O., and Iwata, S. (2004). Crystallization and preliminary X-ray analysis of pseudo-merohedrally twinned crystals of the full-length beta(2) subunit of the Kv1 K(+) channel from Rattus norvegicus. Acta Crystallogr D Biol Crystallogr 60, 912-914.