NICB

Biographical Details:

I obtained a Technicians Diploma in Applied Science (Applied Biology) and a Graduateship of the Institute of Biology (Biochemistry) at Kevin Street, Dublin Institute of Technology. I subsequently obtained a Ph.D. Cell Biology at Dublin City University. My Ph.D. was focused on Lung cell differentiation. I am currently the cell characterisation manager at the National Institute for Cellular Biotechnology, Dublin City University. My research interests have focused on establishing a corneal-limbal stem cell culture techniques for corneal epithelial replacement in Ireland for the first time in conjunction with collaborators in The Royal Victoria Eye & Ear Hospital, Dublin and the Irish Blood Transfusion Service (IBTS). This is currently technique is currently undergoing GLP/GMP evaluation at the IBTS. Current research interests include: ¿ Identifying and deciphering the interactions of limbal - corneal stem cells with the surrounding environment. ¿ Development of methods of culturing other cells of the cornea.

Research Interests:

Stem cells, control of differentiation and Tissue engieering. In particular the cell culture of Cornea-Limbal stem cells and control of differentiaton. Cell characterisation techniques such as confocal mircroscopy, and flow cytometry.

Selected Peer Reviewed Journals

• Dr Finbarr J O'Sullivan. .
• Denis Collins N O Donovan PMcGowan F O¿Sullivan MJ Duffy J Crown. 2012. Trastuzumab induces antibody-dependent cell-mediated cytotoxicity (ADCC) in HER-2 non-amplified breast cancer cell lines. Annals Of Oncology,
• Barron N, Kumar N, Sanchez N, Doolan P, Clarke C, Meleady P, O¿Sullivan F, Clynes M. 2011. Engineering CHO cell growth and recombinant protein productivity by overexpression of miR‐7.. Journal Of Biotechnology, 151, 2, pp204-211.
• Clarke C, Doolan P, Barron N, Meleady P, O'Sullivan F, Gammell P, Melville M, Leonard M, Clynes M. 2011. Predicting cell-specific productivity from CHO gene expression. Journal Of Biotechnology, 151, 2, pp159-165.
• Clarke C, Doolan P, Barron N, Meleady P, O'Sullivan F, Gammell P, Melville M, Leonard M, Clynes M.. 2011. Large scale microarray profiling and coexpression network analysis of CHO cells identifies transcriptional modules associated with growth and productivity. Journal Of Biotechnology, 155, 3, pp350-359.
• McKiernan E, O'Driscoll L., Kasper M., Barron N., O'Sullivan F., Clynes M.. 2007. Directed Differentiation of Mouse Embryonic Stem Cells into Pancreatic-Like or Neuronal- and Glial-Like Phenotypes. Tissue Engineering, 13, 10, pp2419-2430.
• O¿Sullivan F and Clynes M. 2007. Limbal stem cells a review of their identification and culture for clinical use. Cytotechnology, 53, 1-3, pp101-106.
• McKiernan E., Barron N., O¿Sullivan F., Barham P., Clynes M., O'Driscoll L.. 2007. Directing De Novo insulin synthesis in embryonic stem cell derived populations. Experimental Cell Research, 313, 7
• Dowling P, O'Driscoll L, O'Sullivan F, Dowd A, Henry M, Jeppesen PB, Meleady P, Clynes M. 2006. Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress. Proteomics, 6, 24, pp6578-6587.
• Moran E., Cleary I., Larkin A-M., Nic Amhlaoibh R., Masterson A., Scheper R.J., Izquierdo M.A., Center M., O'Sullivan F.,Clynes M. 1997. Co-expression of MDR-associated markers, including P-170, MRP and LRP and cytoskeletal proteins, in three resistant variants of the human ovarian carcinoma cell line, OAW42. European Journal Of Cancer, 33, 4, pp652-660.
• Moran E., Cleary I., Izquierdo M., Scheper R.J., O¿Sullivan F., Gilvarry U., Clynes M. 1995. Preliminary Studies Comparing an Intrinsically Low-Level Cell Line, OAW42-SR and a Low Level, Doxorubicin-Selected Resistant Variant, OAW42-A1 Stable in the Absence of Drug. Journal Of Experimental & Clinical Cancer Research, 14, 1, pp58-60.

Selected Books

• Dr Finbarr J O'Sullivan.

Selected Chapters

• Kishore Reddy and Finbarr O¿Sullivan. 2011. Immunohistochemical and Immunofluorescence Procedures for Protein Analysis. . Gene Expression Profiling : Methods and Protocols Series: Methods in Molecular Biology , pp155-167.
• Meleady P., O' Sullivan F., McBride S., Clynes M.. 1998. Isolation, Cultivation and Differentiation of Lung Type II Epithelial Cells. Animal Cell Culture Techniques, Martin Clynes (ed.), Springer Lab Manual., pp357-370.
• O' Sullivan F., Meleady P., McBride S., Clynes M.. 1998. Primary Culture. Animal Cell Culture Techniques, Martin Clynes (ed.). Springer Lab Manual, pp115-128.

• O'Sullivan F, Establishing cells in culture, considerisations and characterisation., Podocytes In Vitro International Conference, 07-JUN-07 - 09-JUN-07, Dublin, Ireland
• Grabowska J., O¿Sullivan F., Clynes M., McGlynn E., Bio-Applications of ZnO Nanorods-Living cell immobilisation on ZnO Nanorods, One-Day Workshop on Plasma Process for Biomedical Applications, 24-MAY-07 - 24-MAY-07, Dublin Ireland
• Bobart A.S., O¿Sullivan F.,Reddy K., Clynes M., Shaw S., Power W.J.., The Culture Of Corneal Epithelial Tissue From Donor Limbal Stem Cells, Irish College of Ophthalmologists Meeting, 16-MAY-07 - 18-MAY-07, Ireland
• O'Sullivan F., Cornea Epithelial Stem Cells, Global mRNA and Protein Expression Analysis: Research applications in Cancer and other diseases and in biopharmaceutical production, 07-SEP-06 - 08-SEP-06, Dublin, Ireland
• O'Sullivan F., Stem Cells: An overview of our current knowledge and their future potential., Institute of Biology, Annual Meeting, 25-MAY-05 - 25-MAY-05, Dublin, Ireland
• O'Sullivan F., Gammell P., O'Driscoll L., Clynes M., Regulated Differentiation Of Regulated Differentiation Of Murine Teratocarcinoma Cells To Cells Expressing Pancreatic Islet Proteins., 9th International Congress of the International Pancreas and Islet Transplant Association, 09-JUL-03 - 11-JUL-03, Dublin, Ireland
• O' Sullivan, F. and Clynes M., Induction of the Cell Adhesion Protein, Ep-CAM, in Epithelial Cell Lines by Bromodeoxyuridine., Basic Mechanisms of Lung Development, 29-OCT-00 - 01-NOV-00, Woods Hole, MA, USA