Biotechnology

Biographical Details:

Senior Lecturer, School of Biotechnology DCU 2006; Visiting Assist. Professor, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University Baltimore USA 2005-2006; Lecturer, School of Biotechnology DCU 1996-2005; Assistant Lecturer, School of Biotechnology DCU 1992-1996; Post-Doctoral fellow, Institut Gustave-Roussy, Paris, France, 1988-1992 PhD Student, NUI Galway, 1984-1988

Research Interests:

Molecular virology, Epstein-Barr virus and associated cancer biology, Notch signaling, microbial diagnostics and genomics.

Selected Peer Reviewed Journals

• Campion EM, Loughran ST and Walls D. 2011. Protein quantitation and analysis of purity. METHODS IN MOLECULAR BIOLOGY. 681, , pp229-258.
• Guha S, Cullen JP, Morrow D, Colombo A, Lally C, Walls D, Redmond EM and Cahill PA. 2011. Glycogen Synthase Kinase 3 beta (GSK-3ß) positively regulates Notch signaling in vascular smooth muscle cells: role in cell proliferation and survival. BASIC RESEARCH IN CARDIOLOGY. 106, 5, pp773-985.
• Loughran ST and Walls D. 2011. Purification of polyhistidine-tagged proteins. METHODS IN MOLECULAR BIOLOGY. 681, , pp311-335.
• Walls D and Loughran ST. 2011. Tagging recombinant proteins to enhance solubility and aid purification. METHODS IN MOLECULAR BIOLOGY. 681, , pp151-175.
• Walls D, McGrath R and Loughran ST. 2011. A digest of protein purification. METHODS IN MOLECULAR BIOLOGY. 681, pp3-23.
• Loughran ST, Campion EM, D'Souza BN, Smith SM, Vrzalikova K, Wen K, Murray PG, and Walls D. 2011. Bfl-1 is a crucial pro-survival Nuclear Factor-κB target gene in Hodgkin/Reed-Sternberg cells. International Journal Of Cancer, doi: 10.1002/ijc.25950,
• Guha S, Walls D, Redmond EM and Cahill PA. 2010. Pathogenic Mechanisms in Alagille Syndrome. ENCYCLOPEDIA OF LIFE SCIENCES. 10.1002/9780470015902.a0021440 (Review),
• Malizia AP, Lacy N, Walls D, Egan JJ and Doran PP. 2009. CUX/Wnt1 signaling regulates Epithelial Mesenchymal Transition in Epstein-Barr virus-infected epithelial cells. Experimental Cell Research, 11, , pp1819-1831.
• Morrow D, Cullen JP, Liu W, Guha S, Sweeney C, Birney YA, Collins N, Walls D, Redmond EM and cahill PA. 2009. Sonic Hedgehog Activates Notch Signaling in Vascular Smooth Muscle Cells via VEGF-A. Arteriosclerosis Thrombosis And Vascular Biology, 29, , pp1112-1118.
• Malizia AP, Keating DT, Smith SM, Walls D, Doran PP and Egan JJ. 2008. Alveolar Epithelial Cell Injury with Epstein-Barr virus Upregulates TGF-beta-1 Expression. American Journal Of Physiology, 3, , pp451-460.
• Malizia AP, Walls D, Egan JJ and Doran PP. 2008. Viral Infection Drives Tissue Fibrosis In Vitro EUROPEAN RESPIRATORY REVIEWS. 17, 107, pp49-50.
• Morrow D, Guha S, Sweeney C, Birney Y, Walshe T, O'Brien C, Walls D, Redmond EM, Cahill PA. 2008. Notch and vascular smooth muscle cell phenotype CIRCULATION RESEARCH. 103, pp1370-1382.
• Allan GM, McNeilly F, McMenamy M, McNair I, Krakowka SG, Timmusk S, Walls D, Donnelly M, Minihan D, Ellis J, Wallgren P and Fossum C. 2007. Temporal distribution of Porcine Circovirus 2 genogroups recovered from Postweaning Multsystemic Wasting Syndrome affected and non-affected farms in Ireland and Northern Ireland JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. 19, 6, pp668-673.
• Martin HM, Lee JM, Walls D. and Hayward SD. 2007. Manipulation of the Toll-Like receptor signaling pathway by Epstein-Barr virus. Journal Of Virology, 81, 18, pp9748-9758.
• Morrow D, Sweeney C, Birney YA, Guha S, Cummins P, Murphy R, Walls D, Redmond EM, and Cahill PA. 2007. Biomechanical Regulation of Hedgehog Signaling in Vascular Smooth Muscle Cells in Vitro and in Vivo. American Journal Of Physiology, 292, 1, pp488-496.
• Morrow D, Sweeney C, Guha S, Walshe T, O'Brien C, Walls D, Redmond E. and Cahill PA. 2007. Notch signalling and vascular cell fate -how vascular cells respond to stress Wiener Klinische Wochenschrift. 119, , pp7-10.
• Keating DT, Sadlier D, Patricelli A, Smith SM., Walls D, Egan JJ and Doran PP. 2006. Microarray identifies ADAM family members as key responders to TGF-B1 in alveolar epithelial cells. Respiratory Research, 7, 114, pp1-16.
• Loughran ST, Loughran NB, Ryan BJ, D¿Souza BN and Walls D. 2006. Modifed His-tag fusion vector for enhanced protein purifcation by immobilized metal affnity chromatography. Analytical Biochemistry, 355, , pp148-151.
• McMahon KJ, Minihan D, Campion EM, Loughran ST, Allan G, McNeilly F. and Walls D. 2006. Infection of pigs in Ireland with lymphotropic gamma-herpesviruses and relationship to postweaning multisystemic wasting syndrome. Veterinary Microbiology, 116, , pp60-68.
• Morrow D, Sweeney C, Birney YA, Cummins PM, Walls D, Redmond EM, Cahill PA. 2006. Cyclic strain inhibits Notch receptor signaling in vascular smooth muscle cells in vitro CIRCULATION RESEARCH. 96, 5, pp567-575.
• Pegman PM, Smith SM, D¿Souza BN, Loughran ST, Maier S, Kempkes B, Cahill PA, Simmons MJ, Gélinas C and Walls D. 2006. Epstein-Barr Virus Nuclear Antigen 2 trans-Activates the Cellular Antiapoptotic bfl-1 Gene by a CBF1/RBPJ -Dependent Pathway. Journal Of Virology, 80, 16, pp8133-8144.
• Morrow D, Scheller A, Birney YA, Sweeney C, Guha S, Cummins PM, Murphy R, Walls D, Redmond EM, Cahill PA. 2005. Notch-mediated CBF-1/RBP-J{kappa}-dependent regulation of human vascular smooth muscle cell phenotype in vitro. American Journal Of Physiology, 289, 5, pp1188-1196.
• BN. D'Souza, LC. Edelstein, P. Pegman, S. Smith, S. Loughran, A. Clarke, A. Mehl, M. Rowe, C. Gélinas, and D. Walls. 2004. Nuclear factor kB-dependent activation of the anti-apoptotic bfl-1 gene by the Epstein-Barr virus Latent Membrane Protein 1 and activated CD40 receptor. Journal Of Virology, 78, , pp1800-1816.
• Sweeney C, Morrow D, Birney YA, Coyle S, Hennessy C, Scheller A, Cummins PM, Walls D, Redmond EM, Cahill PA.. 2004. Notch 1 and 3 receptor signaling modulates vascular smooth muscle cell growth, apoptosis, and migration via a CBF-1/RBP-Jk dependent pathway. Faseb Journal, 18, 12, pp1421-1423.
• D'Souza B Rowe M Walls D. 2000. The bfl-1 gene is transcriptionally upregulated by the Epstein- Barr virus LMP1 and its expression promotes the survival of a Burkitt's lymphoma cell line. Journal Of Virology, 74, , pp6652-6658.
• Moore S Gunn M Walls D. 2000. A rapid and sensitive PCR-based diagnostic assay to detect bovine herpesvirus 1 in routine diagnostic submissions. Veterinary Microbiology, 75, , pp145-153.
• Moore S Gunn M Walls D. 2000. Clinical and molecular analyses of bovine herpesvirus 1 (BHV1) isolates associated with disease in cattle in Ireland 1971- 1992. Irish Veterinary Journal, 53, , pp89-93.