NICB

Research Interests:

¿Investigating proteins involved in cancer invasion/metastasis; Biomarker discovery ¿Understanding molecular mechanisms involved in diabetes. ¿General Proteomics: LC-MS (Ion Trap), MALDI-ToF, 2D Electrophoresis, Difference In-Gel Electrophoresis (DIGE), SILAC, iTRAQ ¿Immunofluorence, Immunohistochemistry, Western Blotting. ¿Functional Proteomics, Protein Isolation/Purification. ¿Clinical Proteomics: Biomarker Discovery, Surface-Enhanced Laser Desorption Ionization - Time of Flight Mass Spectrometry (SELDI ToF MS).

Selected Peer Reviewed Journals

• Dowling P, Shields W, Rani S, Meleady P, Henry M, Jeppesen P, O'Driscoll L, Clynes M. 2008. Proteomic analysis of conditioned media from glucose responsive and glucose non-responsive phenotypes reveals a panel of secreted proteins associated with beta cell dysfunction. Electrophoresis, 29, 20, pp4141-4149.
• Dowling P, Walsh N, Clynes M. 2008. Membrane and membrane-associated proteins involved in the aggressive phenotype displayed by highly invasive cancer cells. Proteomics,
• Dowling P, Wormald R, Meleady P, Henry M, Curran A, Clynes M. 2008. Analysis of the saliva proteome from patients with head and neck squamous cell carcinoma reveals differences in abundance levels of proteins associated with tumour progression and metastasis. Journal Of Proteomics,
• Walsh N, Dowling P, O'Donovan N, Henry M, Meleady P, Clynes M. 2008. Aldehyde dehydrogenase 1A1 and gelsolin identified as novel invasion-modulating factors in conditioned medium of pancreatic cancer cells. Journal Of Proteomics,
• Kumar N, Maurya P, Gammell P, Dowling P, Clynes M, Meleady P. 2008. Proteomic profiling of secreted proteins from CHO cells using Surface-Enhanced Laser desorption ionization time-of-flight mass spectrometry. Biotechnology Progress, 24, 1, pp273-278.
• Doran P, Martin G, Dowling P, Jockusch H, Ohlendieck K. 2007. Proteome analysis of the dystrophin-deficient MDX diaphragm reveals a drastic increase in the heat shock protein cvHSP. Proteomics, 6, , pp4610-4621.
• Dowling P, Maurya P, Meleady P, Glynn SA, Dowd AJ, Henry M, Clynes M. 2007. Purification and identification of a 7.6-kDa protein in media conditioned by superinvasive cancer cells. Anticancer Research, 27, pp1309-1317.
• Dowling P, Meleady P, Dowd A, Henry M, Glynn S, Clynes M. 2007. Proteomic analysis of isolated membrane fractions from superinvasive cancer cells. Biochimica Et Biophysica Acta-proteins And Proteomics, 1774, pp93-101.
• Maurya P, Meleady P, Dowling P, Clynes M. 2007. Proteomic approaches for serum biomarker discovery in cancer. Anticancer Research, 27, pp1247-1255.
• O¿Connor R, Clynes M, Dowling P, O¿Donovan N, O¿Driscoll L. 2007. Drug resistance in cancer ¿ Searching for mechanisms, markers and therapeutic agents. Expert Opinion On Drug Metabolism And Toxicology,
• Dowling P, O¿Driscoll L, Meleady P, Henry M, Roy S, Ballot J, Moriarty M, Crown J, Clynes M. 2007. Two-dimensional difference gel electrophoresis of the Lung Squamous cell carcinoma versus normal sera demonstrates consistent alterations in the levels of 10 specific proteins. Electrophoresis,
• Dowling P, O'driscoll L, O'sullivan F, Dowd A, Henry M, Jeppesen PB, Meleady P, Clynes M. 2006. Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress. Proteomics, 6, pp6578-6587.