Biotechnology

Biographical Details:

Education
2004 Ph.D. in Chemical Engineering, University of Rome (Italy)
2001 B.Sc./M.Sc. in Chemical Engineering, University of Rome (Italy)

Previous Posts
2005-2008 Postdoctoral Associate at Biotechnology Institute, University of Minnesota (USA)
2005 Junior researcher at Copernicus Institute, Utrecht University (Netherlands)
2003-2004 Visiting scientist at Center for Biofilm Engineering, Montana State University (USA)

Research Interests:

Whole cells biosensors
Microelectrodes for bacterial biofilms
Electrochemical methods for characterization of electron transfer in biofilms
Microbial fuel cells
Biofilm microscopy and image analysis
Heavy metal bioremediation
Life Cycle Engineering

Selected Peer Reviewed Journals

• Das SK, Dickinson C, Lafir F, Brougham DF, and Marsili E. 2012. Synthesis, characterization and catalytic activity of gold nanoparticles biosynthesized with Rhizopus oryzae protein extract. Green Chemistry, 14,, pp1322-1334.
• Das, Sujoy; Liang, Jinni; Schmidt, Michael; Laffir, Fathima; Marsili, Enrico. 2012. Biomineralization Mechanism of Gold by Zygomycete Fungi Rhizopous oryzae. Acs Nano,
• Jain A, Zhang X, Pastorella G, O⿿Connolly J, Barry N, Woolley R, Krishnamurthy S, and Marsili E. 2012. Electron transfer mechanism in Shewanella loihica PV- 4 biofilms formed at graphite electrode. Bioelectrochemistry, In press
• Connolly J, Jain A, Pastorella G, Krishnamurthy S, Mosnier JP, and Marsili E. 2011. Zinc oxide and indium tin oxide thin films for the growth and characterization of Shewanella loihica PV-4 electroactive biofilms. Virulence 2, 5, pp479-482.
• Jain A, Gazzola G, Panzera A, Zanoni M, Marsili E. 2011. Visible spectroelectrochemical characterization of Geobacter sulfurreducens biofilms on optically transparent indium tin oxide electrode. Electrochimica Acta, 56,, pp10776-10885.
• Marsili E, Sun J, Bond DR. 2010. Voltammetry and physiology of Geobacter sulfurreducens biofilms as a function of growth stage and imposed potential. Electroanalysis, 22, 7, pp865-874.
• Sujoy K.Das and Enrico Marsili. 2010. A green chemical approach for the synthesis of gold nanoparticles: characterization and mechanistic aspect. Reviews in Environmental Science and Bio/Technology 9, 3, pp199-204.
• Aldrovandi, A., Marsili, E., Stante, L., Paganin, P., Tabacchioni,S., and Giordano, A. 2009. Sustainable power production in a membrane-less and mediator-less synthetic wastewater microbial fuel cell. Bioresource Technology, 100, 13, pp3252-3260.
• Marsili, E., Rollefson, J.B., Hozalski, R., and Bond, D.R. 2008. Microbial biofilm voltammetry: direct electrochemical characterization of catalytic electrode-attached biofilms. Applied And Environmental Microbiology, 74, 23, pp7329-7737.
• Marsili, E., Baron, D.B., Shikhare, I., Coursolle, D., Gralnick, J.A., and Bond, D.R. 2008. Shewanella secretes flavins that mediate extracellular electron transfer. Proceedings Of The National Academy Of Sciences Usa (pnas), 105, 10, pp3968-3973.
• Shrikant, S., Marsili, E., Flickinger, M.C., and Bond, D.R. 2008. Electrochemical characterization of Geobacter sulfurreducens cells immobilized on graphite paper electrodes. Biotechnology And Bioengineering, 1099, 5, pp1065-1073.
• Roes A.L., Marsili E., Nieuwlaar E. and Patel M.K. 2007. Environmental and cost assessment of nanoclay-polypropylene composite. Journal of Polymers and Environment 15, , pp212-226.
• Marsili, E., Beyenal, H., Di Palma, L., Merli, C., Dohnalkova, A., Amonette, J.E., and Lewandowski, Z. 2007. Uranium immobilization using sulfate reducing biofilms grown on hematite, dolomite, and calcite. Environmental Science And Technology, 41, 24, pp8349-8354.
• Menicucci, J., Beyenal, H., Marsili, E., Veluchamy, R. R. A., Demir, G., and Lewandowski, Z. 2006. Procedure for determining maximum sustainable power generated by microbial fuel cells. Environmental Science And Technology, 40, 3, pp1062-1068.
• Marsili, E., Beyenal, H., Di Palma, L., Merli, C., Dohnalkova, A, Amonette, J.E., and Lewandowski, Z. (2005). 2005. Uranium removal by sulphate-reducing biofilms in the presence of carbonates. Water Science And Technology, 52, 7, pp49-55.
• Fidaleo, M., Lavecchia, R., and Marsili, E. 2001. Enzymatic extraction of carotenoids from plant material using two immiscible liquid phases. 66, 3a, pp295-298.

Selected Chapters

• Jain A, Marsili E, Bhosle NB. 2011. The biofilm returns: microbial life at interface . Microbes and microbial technology, pp59-85.
• Arora S, Pastorella G, Byrne B, Marsili E, and O⿿Kennedy R. 2010. Arora S, Pastorella G, Byrne B, Marsili E, and O⿿Kennedy R (2009) Microbial Cells and Biosensing: A Dual Approach - Exploiting Antibodies and Microbial Cells as Analytical/Power Systems. Reviews in Pharmaceutical and Biomedical Analysis (E-book series).
• Marsili, E., Zhang, X. 2009. Shuttling via soluble compounds. Bio-electrochemical Systems: from extracellular electron transfer to biotechnological application

Selected Non-peer Reviewed Journals

• Marsili, E. and Frankl, P. 2004. Marsili, E. and Frankl, P. (2004) Greenhouse gas emissions in the life cycle of paper and aluminium foil consumer products. Attenzione, 2, pp7-11.

• Marsili E., Beyond graphite: novel nanostructured interfaces for electroactive biofilms characterization., Annual Meeting of Society for Industrial Microbiology, 24-JUL-11 - 28-JUL-11, New Orleans
• Marsili E., Murphy, P., A dialogic life cycle approach to nanotechnology?, VI Celsius Symposium on Science and Society., 10-JUN-11 - 10-JUN-11, Dublin
• Jain A., Pastorella G., Barry N., Liu Y., Fattakhova-Rohlfing D., and Marsili E., Porous indium tin oxide electrodes for enhanced current production by Shewanella loihica PV-4 biofilms., BioElectrochemical Society (BES) XXI International Symposium on Bioelectrochemistry and Bioenergetics,, 08-MAY-11 - 12-MAY-11, Krakow (Poland)
• Zhang, X. and Marsili, E., Electrochemical characterization of shewanella biofilms grown on high surface carbon electrodes., BioElectrochemical Society (BES) XXI International Symposium on Bioelectrochemistry and Bioenergetics., 08-MAY-11 - 12-MAY-11, Krakow (Poland)
• Das SK, Marsili E., Microbial Protein Mediated One Pot Synthesis of Gold Nanoparticles, INDO - ITALIAN WORKSHOP ON BACTERIA & FUNGI FOR ENVIRONMENTAL SUSTAINABILITY, 29-NOV-10 - 01-DEC-10, New Delhi (India)
• Jain A, Gazzola G, Connolly J, Krishnamurthy S, Mosnier JP, Marsili E , Spectroelectrochemistry of electroactive biofilms, 61st International Society of Electrochemistry Annual Meeting, 26-SEP-10 - 01-OCT-10, Nice (France)
• Pastorella G, Marsili E., Genetic technology to improve biofilms electroactivity for bioremediation applications, 61st International Society of Electrochemistry Annual Meeting, 26-SEP-10 - 01-OCT-10, Nice (France)
• Marsili E, Novel materials and methods for characterization of electroactive biofilms, 61st International Society of Electrochemistry Annual Meeting, 26-SEP-10 - 01-OCT-10, Nice (France)
• Connolly J, McCarthy E, Gazzola G, Jain A, Krishnamurthy S, Marsili E, Mosnier J-P., Laser-produced thin layers for viable electroactive biofilms (EAB) analysis, European Material Research Society Spring Meeting, 07-JUN-10 - 11-JUN-10, Strasbourg (France)
• Marsili, E., Baron, D.B., and Bond, D.R., Insights from non-turnover cyclic voltammetry and rotating disk electrode studies of electrode-reducing bacteria., 108th American Society for Microbiology (ASM) general meeting., 01-JUN-08 - 05-JUN-08, Boston (MA), USA
• Harrington, C.P., Marsili, E., Shimotori, T., LaPara, T.M., Hozalski, R.M., Bond, D.R., The effect of inoculation source on the structure and function of bacterial communities in microbial fuel cells., 108th ASM general meeting, 01-JUN-08 - 05-JUN-08, Boston (MA), USA
• Shimotori, T., Marsili, E., Bond, D.R., LaPara, T.M., and Hozalski, R.M., A model of power production by Geobacter spp. at fuel cell anodes., Microbial Fuel Cells first international symposium, 27-MAY-08 - 29-MAY-08, Penn State University (PA), USA
• Marsili, E., Harrington, C.P., Shimotori, T., Hozalski, R.M., LaPara, T. M., Bond, D.R., Electrochemical characterization of mixed species biofilms grown in microbial fuel cells., 213th Electrochemical Society meeting, 21-MAY-08 - 25-MAY-08, Phoenix (AZ), USA
• Marsili, E., Harrington, C.P., Hozalski, R.M., LaPara, T. M., Cussler, E.L., and Bond, D.R., An integrated approach for optimization of microbial fuel cells., E3 2007 Conference., 27-NOV-07 - 27-NOV-07, Minneapolis (MN), USA
• Shimotori, T., Marsili, E., Hozalski, R.M, Bond, D.R., and LaPara, T.M., The impact of external resistance on the physiology and bacterial community composition of microbial fuel cells., Annual meeting of the North-Central Branch of the American Society of Microbiology, 05-OCT-07 - 06-OCT-07, Marshfield, (WI), USA
• Wee, W., Marsili, E., Zhuang, K., Mahadevan, R., A glucose utilizing co-colture microbial fuel cell., UNERD Conference, 29-AUG-07 - 29-AUG-07, Toronto (Canada)
• Shimotori, T., Marsili, E., Drozdowicz, M.P., Bond, D.R., Hozalski, R.M., and LaPara, T.M., Electrochemical and molecular-biological analyses of microbial fuel cells inoculated with anaerobic digester sludge., 234th American Chemical Society (ACS) National Meeting,, 19-AUG-07 - 23-AUG-07, Boston (MA), USA
• Coursolle, D., Baron, D.B., Marsili, E., Bond, D.R., Gralnick, J.A., Shuttling happens: identification and physiological characterization of a small molecule involved in electron transfer to surfaces., Gordon Research Conference on Applied and Environmental Microbiology, 15-JUL-07 - 20-JUL-07, Mount Holyoke College (MA), USA
• Baron, D.B., Marsili, E., Gralnick, J.A., and Bond, D.R., Electrochemical characterization of Shewanella biofilms on electrodes., 107th American Society for Microbiology general meeting,, 21-MAY-07 - 25-MAY-07, Toronto (Canada)
• Marsili, E., Baron, D.B., and Bond, D. R., Techniques for electrochemical characterization of electricity-producing bacteria., 107th American Society for Microbiology general meeting, 21-MAY-07 - 25-MAY-07, Toronto (Canada)
• Werner, L., Marsili, E., Alley, J., and Bond, D.R., Detecting early colonization of electrodes using microfabricated gold electrodes., 107th Anerican Society for Microbiology general meeting,, 21-MAY-07 - 25-MAY-07, Toronto (Canada)
• Marsili, E. and Bond, D.R., Electrochemical methods for observing electron transfer from bacterial biofilms to conductive surfaces., 211th Electrochemical Society Meeting., 06-MAY-07 - 11-MAY-07, Chicago (IL), USA
• Marsili, E., Shimotori, T., Hozalski, Hozalski, R.M., LaPara, T.M., and Bond, D.R., An integrated approach for optimization of microbial fuel cells (MFCs)., Third Annual IREE Symposium., 28-NOV-06 - 28-NOV-06, Minneapolis (MN), USA
• Shimotori, T., Marsili, E., Bond, D. R., Hozalski, R.M., and LaPara, T.M., Comprehensive modeling of a microbial fuel cell., AIChE Annual Meeting,, 12-NOV-06 - 17-NOV-06, San Francisco (CA), USA
• Roes Lex, E. Marsili, E. Nieuwlaar, Patel, M.K., Environmental and cost assessment of nanoclay-polypropylene composite., Nanofun-Poly International Conference,, 29-MAY-06 - 31-MAY-06, Villeurbanne (France),
• Shrikant, S., Marsili, E., Flickinger, M.C., and Bond. D.R., Preparation of adhesive electrochemically reactive coatings using immobilized Geobacter sulfurreducens., 106th American Society for Microbiology general meeting., 21-MAY-06 - 25-MAY-06, Orlando (FL), USA
• Hunt, B., Marsili, E., Bond, D.R., Microfabrication of anodes for use in high-throughput bioelectrochemistry., 2nd ASM - IEEE EMBS Conference on Bio, Micro and Nanosystems., 12-NOV-05 - 15-NOV-05, San Francisco (CA), USA
• Menicucci, J.A., Beyenal, H., Marsili, E. Veluchamy, R.R.A., and Lewandowski, Z., Sustainable power measurement for a microbial fuel cell., 2005 AIChE annual fall meeting,, 30-OCT-05 - 04-NOV-05, Cincinnati (OH), USA
• Marsili, E., Patel, M., and Nieuwlaar, E., Life cycle assessment and life cycle costing for nanomaterial applications., Nanofun-Poly 2005 - 1st International Symposium on Nanostructured and functional polymer-based materials and composites., 24-APR-05 - 27-APR-05, Dresden (Germany)
• Marsili, E., Beyenal, H., Di Palma, L., Merli, C., Dohnalkova, A., Amonette, J.E., and Lewandowski, Z., Immobilizing uranium in sulfate reducing biofilms, Biofilms 2004 international conference, 24-OCT-04 - 26-OCT-04, Las Vegas (NE), USA
• Marsili, E., Beyenal, H., Di Palma, L., Merli, C., and Lewandowski, Z., Determination of uranium concentration in wastewater from sulfate reducing biofilm reactors using voltammetry., Montana State University technical advisory conference,, 05-FEB-04 - 06-FEB-04, Bozeman (MT), USA
• Marsili, E., Beyenal, H., Di Palma, L., Merli, C., and Lewandowski, Z., Biogeochemistry of uranium under reducing and re-oxiding conditions: an integrated laboratory and field study., Montana State University technical advisory conference,, 22-JUL-03 - 24-JUL-03, Bozeman (MT), USA