Biotechnology

Dr Ciaran Fagan

Dr Ciaran Fagan
Name:Dr Ciaran Fagan
Phone Number5288
Department:Biotechnology
Room:X207
E-Mail Address:
Work Area:Head of School

Biographical Details:


Ciaran Fagan (Ciarán Ó'Fágain) Tel. +353 (0)1 7005288; Email ciaran.fagan@dcu.ie * 1978 BA (Mod) in Biochemistry, H2.1, University of Dublin (Trinity College). * 1982 PhD, University of Dublin (Trinity College). * 1981-4 WBE Ltd, Dublin: microbial biomass production and technical services. * 1984-7 Noctech Ltd, Galway: immunodiagnostics development. * 1987-90 Postdoctoral researcher, temporary lecturer, NIHE Dublin. * 1990 Permanent position, School of Biological Sciences, DCU. * 1997 Published 200-page sole-author book Stabilizing Protein Function, Springer-Verlag, ISBN 3 540 63189 5. * 1999- Member of HEA-funded National Centre for Sensors Research, DCU. * 2004 Promoted to Senior Lecturer. * 2007-2011 Editorial Board Member, Enzyme and Microbial Technology. * 2008-2011 Editorial Board Member, Journal of Molecular Catalysis B: Enzymatic. Total Graduations and Publications: 9 PhD, 2 MSc Graduates. 34 peer-reviewed papers, 8 refereed reviews (including 2 invited articles), 1 sole-author book, 10 book chapters, 2 patent applications, 8 conference/ other papers.


Research Interests:


Stability and stabilization of enzymes and proteins, chemical modification of proteins, protein engineering, peroxidases, trypsin, enzymatic peptide synthesis. View some publications at: http://www.ir.dcu.ie/view/author/=D3=27F=E1g=E1in,_Ciar=E1n.html


Selected Peer Reviewed Journals


  • Dr Ciarán Fagan [Ó'Fágáin]. .
  • Loughran NB, O'Connell MJ, O'Connor B, Ó'Fágáin C. 2014. Stability properties of an ancient plant peroxidase. Biochimie, 104,, pp156-159.
  • Loughran, NB; Hinde, S; McCormick-Hill, S; Leidal, KG; Bloomberg, S; Loughran, ST; O¿Connor, B; Ó´Fágáin, C; Nauseef WJ; O¿Connell, MJ. 2012. Functional Consequence of Positive Selection Revealed Through Rational Mutagenesis of Human Myeloperoxidase. Molecular Biology And Evolution, 29, 8, pp2039-2046.
  • McEntee G, Minguzzi S, O¿Brien K, Ben Larbi N, Loscher C, Ó¿Fágáin C, & Parle-McDermott A. 2011. The former annotated human pseudogene dihydrofolate reductase-like 1 (DHFRL1) is expressed and functional. National Academy Of Sciences. Proceedings (print), 108,, pp15157-15162.
  • Forde J, Tully E, Vakurov A, Gibson TD, Millner P, Ó¿Fágáin C. 2010. Forde J, Tully E, Vakurov A, Gibson TD, Millner P, Chemical modification and immobilisation of laccase from Trametes hirsuta and from Myceliophthora thermophila. Enzyme And Microbial Technology, 46:, , pp430-437.
  • Forde J, Vakurov A, Gibson TD, Millner P, Whelehan M, Marison IW, Ó¿Fágáin C. 2010. Chemical modification and immobilisation of lipase B from Candida antarctica onto mesoporous silicates. Journal Of Molecular Catalysis B-enzymatic, 66:, , pp203-209.
  • Sandra COLLEARY & Ciarán Ó'FÁGÁIN. 2009. Stability and catalytic properties of chemically modified pig trypsin. Biocatalysis And Biotransformation, 27, , pp309-317.
  • Barry J. Ryan , Ciar¿¿n ¿¿'F¿¿g¿¿in. 2008. Effects of mutations in the helix G region of horseradish peroxidase. Biochimie, 90,, pp1414-1421.
  • Barry J. Ryan , Mary J. O'Connell , Ciar¿¿n ¿¿'F¿¿g¿¿in. 2008. Consensus mutagenesis reveals that non-helical regions influence thermal stability of horseradish peroxidase. Biochimie, 90,, pp1389-1396.
  • Barry J RYAN, Ciarán Ó'FÁGÁIN. 2008. Effects of Mutations in the Helix G Region of Horseradish Peroxidase. Biochimie, 90, , pp1414-1421.
  • Barry J RYAN, Mary J O'CONNELL, Ciarán Ó'FÁGÁIN. 2008. Consensus Mutagenesis Reveals that Non-Helical Regions Influence Thermal Stability of Horseradish Peroxidase. Biochimie, 90, , pp1389-1396.
  • Deborah M RUTH, Seamus J BUCKLEY, Brendan F O'CONNOR, Ciarán Ó'FÁGÁIN. 2007. Solvent and Thermal Stability, and pH Kinetics, of Proline-specific Dipeptidyl Peptidase IV-like Enzyme from Bovine Serum. Enzyme And Microbial Technology, 41, , pp307-311.
  • Neil CAROLAN, Robert J FORSTER, Ciarán Ó'FÁGÁIN. 2007. Covalent attachment of ferrocene to soybean peroxidase glycans: electron transfer mediation to redox enzymes. Bioconjugate Chemistry, 18, , pp524-529.
  • Barry J. Ryan , Ciar¿¿n ¿¿'F¿¿g¿¿in. 2007. Arginine-to-lysine substitutions influence recombinant horseradish peroxidase stability and immobilisation effectiveness. Bmc Biotechnology, 7,
  • Ryan B.J. & Ó'Fágáin C. 2007. Arginine-to-lysine substitutions influence recombinant horseradish peroxidase stability and immobilisation effectiveness. Bmc Biotechnology, 7, , pp86-.
  • Barry J. Ryan , Ciar¿¿n ¿¿'F¿¿g¿¿in. 2007. Effects of single mutations on the stability of horseradish peroxidase to hydrogen peroxide. Biochimie, 89,, pp1029-1032.
  • Barry J RYAN, Ciarán Ó'FÁGÁIN. 2007. Effects of single mutations on the stability of horseradish peroxidase to hydrogen peroxide. Biochimie, 89, 8, pp1029-1032.
  • Deborah M RUTH, Gillian McMAHON & Ciarán Ó'Fágáin. 2006. Peptide synthesis by recombinant Fasciola hepatica cathepsin L1. Biochimie, 88, , pp117-120.
  • Barry J RYAN, Neil CAROLAN & Ciarán Ó'FÁGÁIN. 2006. Horseradish and soybean peroxidases: comparable tools for alternative niches?. Trends In Biotechnology (tibtech), 24, 8, pp355-363.
  • Barry J. Ryan , Neil Carolan , Ciar¿¿n ¿¿'F¿¿g¿¿in. 2006. Horseradish and soybean peroxidases: comparable tools for alternative niches?. Trends In Biotechnology (tibtech), 24,, pp355-363.
  • Deborah M. Ruth , Gillian McMahon , Ciar¿¿n ¿¿'F¿¿g¿¿in. 2006. Peptide synthesis by recombinant Fasciola hepatica cathepsin L1. Biochimie, 88,, pp117-120.
  • A-M O'Brien, AT Smith & C Ó'Fágáin. 2003. Effects of Phthalic Anhydride Modification on Horseradish Peroxidase Stability and Activity. Biotechnology And Bioengineering, 81,, pp233-240.
  • A-M O'Brien, C Ó'Fágáin, PF Nielsen & KG Welinder. 2001. Location of Crosslinks in Chemically Stabilized Horseradish Peroxidase. Implications for Design of Crosslinks. Biotechnology And Bioengineering, 76,, pp277-284.
  • Dowd AJ Dooley M Ó'Fágáin C Dalton JP. 2000. Stability studies on the cathepsin L proteinase of the helminth parasite, Fasciola hepatica. Enzyme And Microbial Technology, 27,, pp599-604.
  • O'Brien A-M Ó'Fágáin C. 2000. Dye bleaching and phenol precipitation by phthalic anhydride-modified horseradish peroxidase. Journal Of Chemical Technology And Biotechnology, 75,, pp363-368.
  • A Murphy & C Ó'Fágáin. 1998. Chemically stabilized trypsin used in dipeptide synthesis. Biotechnology And Bioengineering, 58,, pp366-373.
  • A Murphy & C Ó'Fágáin. 1997. Dipeptide synthesis using acetylated trypsin derivative. Biotechnology Techniques, 11,, pp13-16.
  • J Moreno & C Ó'Fágáin. 1997. Activity and stability of native and modified alanine aminotransferase in cosolvent systems and denaturants. Journal Of Molecular Catalysis B-enzymatic, 2,, pp271-279.
  • A Murphy & C Ó'Fágáin. 1996. Stability characteristics of chemically modified soluble trypsin. Journal Of Biotechnology, 49,, pp163-171.
  • A-M O'Brien & C Ó'Fágáin. 1996. Chemical stabilization of recombinant horseradish peroxidase. Biotechnology Techniques, 10,, pp905-910.
  • E Miland, MR Smyth & C Ó'Fágáin. 1996. Phenol removal by modified peroxidases. Journal Of Chemical Technology And Biotechnology, 67,, pp227-236.
  • E Miland, MR Smyth & C Ó'Fágáin. 1996. Modification of horseradish peroxidase with bifunctional N-hydroxysuccinimide esters: effects on molecular stability. Enzyme And Microbial Technology, 19,, pp242-249.
  • E Miland, MR Smyth & C Ó'Fágáin. 1996. Increased thermal and solvent tolerance of acetylated horseradish peroxidases. Enzyme And Microbial Technology, 19,, pp63-67.
  • J Moreno & C Ó'Fágáin. 1996. Immobilization of alanine aminotransferase by covalent binding and entrapment. Biotechnology And Applied Biochemistry, 23,, pp231-235.
  • J Moreno & C Ó'Fágáin. 1996. Stabilization of alanine aminotransferase by consecutive modification and immobilization. Biotechnology Letters, 18,, pp51-56.
  • C Besson, G Favre-Bonvin, C Ó'Fágáin & JM Wallach. 1995. Chemical derivatives of Pseudomonas aeruginosa elastase showing increased stability. Enzyme And Microbial Technology, 17,, pp877-881.
  • F Manning, C Ó'Fágáin, R O'Kennedy & B Woodhams. 1995. Effects of chemical modifiers on recombinant Factor VIII activity. Thrombosis Research, 80,, pp247-254.
  • O Ryan, MR Smyth & C Ó'Fágáin. 1994. Thermostabilized chemical derivatives of horseradish peroxidase. Enzyme And Microbial Technology, 16,, pp501-505.
  • C Ó'Fágáin, R O'Kennedy & CG Kilty. 1991. Stability of alanine aminotransferase is enhanced by chemical modification. Enzyme And Microbial Technology, 13,, pp234-239.
  • A Shattock, M Morris, K Kinane & C Fagan. 1989. The serology of delta hepatitis and the detection of IgM anti-HD by EIA. Journal Of Virology, 23, , pp233-240.
  • C Ó'Fágáin, B Butler & T Mantle. 1983. The effect of pH on the kinetics of arylsulphatase A. Biochemical Journal, 213, 6, pp606-607.
  • C Ó'Fágáin, U Bond, B Orsi & T Mantle. 1982. The slow kinetic transients of arylsulphatase A. Biochemical Journal, 201, , pp345-352.

Selected Books


  • C Ó'Fágáin. Stabilizing Protein Function, Springer Verlag, 1997.

Selected Chapters


  • Dr Ciarán Fagan [Ó'Fágáin]. .
  • Ciarán Ó'Fágáin. 2012. Engineering Protein Stability. Protein Engineering Handbook vol 3, pp115-144., Wiley-VCH
  • Ciarán Ó'Fágáin. 2011. Engineering Protein Stability. Protein Chromatography: Methods and Protocols, Methods in Molecular Biology, vol. 681, pp103-136., Humana Press-Springer
  • Ciarán Ó'Fágáin. 2011. Storage and Lyophilisation of Pure Proteins. In: Protein Chromatography: Methods and Protocols, Methods in Molecular Biology, vol. 681, pp179-202., Humana Press-Springer
  • Ciarán Ó'Fágáin, PM Cummins & BF O¿Connor. 2011. Gel-Filtration Chromatography. Protein Chromatography: Methods and Protocols, Methods in Molecular Biology, vol. 681, pp25-33., Humana Press-Springer
  • K Mtawae, B O'Connor & C Ó'Fágáin. 2008. Stability and Kinetic Studies on Recombinant Human Pyroglutamyl Peptidase 1 and a Single-Site Variant, Y147F. Biotechnology: Research, Technology and Applications, pp121-141., Nova Science Publishers, Inc.
  • S Colleary & C Ó'Fágáin. 2008. Chemically stabilized subtilisins in peptide synthesis. Biotechnology and Bioengineering, pp75-97., Nova Science Publishers, Inc.
  • Sandra Colleary , Ciar¿¿n ¿¿'F¿¿g¿¿in. 2008. Chemically stabilized subtilisins in peptide synthesis. Biotechnology and bioengineering, pp75-97., Nova Science Publishers
  • C Ó'Fágáin. 2004. Storage of Pure Proteins. Protein Purification Protocols, 2nd ed., pp323-337., Humana Press
  • C Ó'Fágáin. 2004. Lyophilization of Proteins . Protein Purification Protocols:2nd edition., pp309-321., Humana Press
  • A Murphy & C Ó'Fágáin. 1996. Bypassing the ribosome: peptide synthesis without translation. Essays in Biochemistry vol 31 :, pp61-75., Portland Press
  • O Ryan, M Smyth & C Ó'Fágáin. 1994. Horseradish peroxidase: the analyst's friend. Essays in Biochemistry vol 28, pp129-146., Portland Press

Reviews


  • BJ Ryan, N Carolan & C Ó¿Fágáin. 2006. Horseradish and soybean peroxidases: comparable tools for alternative niches?. Trends In Biotechnology (tibtech), 24, pp355-363.
  • C Ó'Fágáin. 2003. Enzyme stabilization - recent experimental progress. Enzyme And Microbial Technology, 33, pp137-149.
  • C Ó'Fágáin. 1995. Understanding and increasing protein stability. Biochimica Et Biophysica Acta, 1252, pp1-14.
  • F Manning, C Ó'Fágáin & R O'Kennedy. 1993. Factor VIII: structure, function and analysis. Biotechnology Advances, 11, pp79-114.
  • C Ó'Fágáin & R O'Kennedy. 1991. Functionally stabilized proteins. Biotechnology Advances, 9, pp351-409.
  • C Ó'Fágáin, H Sheehan, R O'Kennedy & C Kilty. 1988. Maintenance of enzyme structure. Process Biochem 23: 166-171. 23, pp166-171.

Selected Non-peer Reviewed Journals


  • Ciaran O'Fagain. 2003. The Membrane Dissected. Review of 'Biophysical Chemistry: membranes and proteins' R Templer & R Leatherbarrow, eds. Cambridge: Royal Society of Chemistry, 2002. Chemistry & Industry, *, pp21-21.
  • Ciaran O'Fagain. 2003. Review of Peptides: chemistry and biology. N Sewald & H-D Jakubke. Weinheim: Wiley-VCH, 2002. http://www.biochemist.org/reviews/page.htm?item=462, *,
  • S Green & C Ó'Fágáin. 1999. Peptide synthesis using modified trypsin. Biochemical Society Transactions, 27, pp727-730.

Conferences:


  • Loughran NB; O'Connell MJ; O'Connor B; Ó'Fágáin, C., Stability properties of an ancient plant peroxidase, ProStab2012: 9th International Conference on Protein Stabilisation, 02-MAY-12 - 04-MAY-12, Lisbon, Portugal
  • Fagan, C., Research and Teaching in DCU's School of Biotechnology, The University and Economic Recovery: Role of Technology, Enterprise and Learning Approaches, 25-OCT-11 - 26-OCT-11, Dublin City University
  • FAGAN, Ciaran, Better enzymes for biosensors, US/Ireland Emerging Technologies Conference, Lowell, MA, USA, Oct 19-20 2009, 19-OCT-09 - 20-OCT-09, UMass Lowell, Lowell, MA, USA
  • B Ryan & C Fagan, Probing peroxidase with new tools and old, ProStab2009, April 14 - 17, 2009 8th International Conference on Protein Stabilisation, 14-APR-09 - 17-APR-09, Graz, Austria
  • B Ryan, N Carolan & C Ó'Fágáin, Some Ups and Downs with Horseradish and Soybean Peroxidases, Protein Engineering Club, Conway Institute, UCD., 30-JAN-09 - 30-JAN-09, Dublin, Ireland
  • Neil Carolan & Ciarán Ó'Fágáin, Chemical Modification of Soybean Peroxidase Glycans, ProStab2004: 6th International Conference on Protein Stabilization, 26-SEP-04 - 29-SEP-04, Bratislava, Slovakia
  • S Colleary & C Ó'Fágáin., Formylation of porcine trypsin., EuropaCat IV, 02-SEP-01 - 06-SEP-01, Limerick, Ireland
  • D O'Brien & C Ó'Fágáin., pH stability of chemically modified horseradish peroxidase., 2nd International Conference on Protein Stabilization/ Biomolecule Stabilization, 09-APR-00 - 12-APR-00, Lisbon, Portugal
  • U McKeon, BF O'Connor & C Ó'Fágáin., Stability properties of calf intestinal alkaline phosphatase. In: Ballesteros A, Plou FJ, Iborra JL & Halling PJ (eds). Stability and Stabilization of Biocatalysts. Elsevier, Amsterdam, 47-52. ISBN 0 444 82970 9, Stability and Stabilization of Biocatalysts. European Federation of Biotechnology Symposium, 19-APR-98 - 22-APR-98, Cordoba, Spain