Registry

Module Specifications

Current Academic Year 2012 - 2013
Please note that this information is subject to change.

Module Title	Gene Cloning and Gene Expression
Module Code	BE301
School	School of Biotechnology
Online Module Resources
Module Co-ordinator	Semester 1: Michael O'Connell Semester 2: Michael O'Connell Autumn: Michael O'Connell
NFQ level	8	Credit Rating	5
Pre-requisite	None
Co-requisite	None
Compatibles	None
Incompatibles	None

Description

To develop a good understanding of the biology of bacteriophages and plasmids. To teach the basis for techiques used in the manipulation of DNA and to provide a theoretical background that would enable students to learn the skills involved in DNA manipulation. To develop an appreciation and understanding of the complexity of gene expression and its control, with an emphasis on eukaryotic systems.

Learning Outcomes

1. Design DNA cloning strategies exploiting the knowledge of restriction enzymes, vector systems and PCR technology
2. Analyse genome sequences to gain an understanding of the genetic organisation of the encoded information
3. Design an experimental approach to monitor the expression of genes using RNA analysis
4. Select the appropriate vector system for application in genetic analysis

Workload	Full-time hours per semester
*Type*	*Hours*	*Description*
Tutorial	4	No Description
Lecture	36	No Description
Independent learning time	85	Reading recommended text books
Total Workload: 125

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

Recombinant DNA technology.
- restriction enzymes, joining DNA molecules. DNA amplification, the polymerase chain reaction. Vectors for gene cloningPlasmids, introduction to plasmids.

- structure, high and low copy number, different forms, plasmid encoded phenotypes.Replication of plasmids.
DNA sequencing methodologies..

Assessment Breakdown
Continuous Assessment	0%	Examination Weight	100%

Course Work Breakdown
Type	Description	% of total	Assessment Date

Reassessment Requirement
Resit arrangements are explained by the following categories; 1 = A resit is available for all components of the module 2 = No resit is available for 100% continuous assessment module 3 = No resit is available for the continuous assessment component
This module is category 1

Indicative Reading List