Registry

Module Specifications

Current Academic Year 2012 - 2013
Please note that this information is subject to change.

Module Title	Biochemical and Microbiological Analysis
Module Code	BE302
School	School of Biotechnology
Online Module Resources
Module Co-ordinator	Semester 1: Brid Quilty Semester 2: Brid Quilty Autumn: Brid Quilty
NFQ level	8	Credit Rating	5
Pre-requisite	None
Co-requisite	None
Compatibles	None
Incompatibles	None

Description

To give students knowledge of: Food and water microbiology. Food biochemistry. Protein purification and analysis. Immunology with particular reference to analysis.

Learning Outcomes

1. Discuss the factors governing the microbiology of food and water
2. Identify the causes of food spoilage and food poisoning
3. Select a suitable method for the microbiological examination of food and water
4. Outline the biochemical composition of all foods
5. Describe the many and varied methods of protein purification
6. Understand the immune system, the methods used in its analysis and how anti-body based tests are of major importance in analysis

Workload	Full-time hours per semester
*Type*	*Hours*	*Description*
Lecture	36	No Description
Tutorial	4	No Description
Independent learning time	85	No Description
Total Workload: 125

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

Factors influencing the types and numbers of microorganisms in foods.
Primary sources of microbes in foodsIntrinsic parameters of food that affect microbial growth Extrinsic Parameters.

Food spoilage.
Spoilage of fruit, vegetables, meat fish and eggsSpoilage of canned foods.

Food preservation.
ChemicalsIrradiationLow temperaturesHigh temperatures.

The microbiological examination of foods.
SamplingMicrobiological examinationInterpretation of results and reporting.

Food poisoning.
Main food poisoning microorganisms.

Water Microbiology.
Water supplyContaminants in waterMicrobiological examination of water.

Food biochemistry.
starch; fats; emulsions; foams; pectic substances and gelatin; composition of meat, cereals and dairy produce.â€¢.

Protein purification and analysis.
Important physiochemical properties of proteins and peptidesâ€¢Genetic manipulation of source of proteinsâ€¢Methods of cellular disruptionâ€¢Column Chromatography Methodsâ€¢Storage and stability of proteins and peptides.

Protein and peptide analysis.
PAGE and HPLC.

Immunology.
introduction to the immune system; innate immunity, antibodies, complement, B-cells, T-cells, macrophages, generation of the immune response, antibody diversity, antibody-antigen interaction and immunoassays..

Assessment Breakdown
Continuous Assessment	0%	Examination Weight	100%

Course Work Breakdown
Type	Description	% of total	Assessment Date

Reassessment Requirement
Resit arrangements are explained by the following categories; 1 = A resit is available for all components of the module 2 = No resit is available for 100% continuous assessment module 3 = No resit is available for the continuous assessment component
This module is category 1

Indicative Reading List

Jay, James M.: 2005, Modern food microbiology, Springer,,
I. Roitt: 2001, Essential Immunology ,, Blackwell Publications,
T.P. Coultate: 0, Food - The Chemistry of Its Components,
S. Roe: 2001, Protein Purification Techniques, Oxford Press,
T J Montville and K R Matthews: 2008, Food Microbiology an Introduction, Washington, D.C. : Oxford : ASM ; Blackwell [distributor,
B Ray: 2004, Fundamental Food Microbiology, Boca Raton, Fl : CRC Press,
Arnold E. Greenberg, Lenore S. Clesceri, Andrew D. Eaton ; Mary Ann H. Franson: 0, Standard methods for the examination of water and wastewater., American Public Health Association,

Other Resources

None

Array

Programme or List of Programmes

BSc in Analytical Science

BSSA

Study Abroad (DCU Business School)

BSSAO

Study Abroad (DCU Business School)

BSc in Biotechnology

ECSA

Study Abroad (Engineering & Computing)