Registry
Module Specifications
Current Academic Year 2012 - 2013
Please note that this information is subject to change.
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| Description | |||||||||||||||||||||||||||||||||||||||||
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This module will introduce students to the growth of microbial and animal cells in culture,the scale up of cells into large scale fermentations and the products produced from them.To introduce students to ways plants may be manipulated in culture for mass propagationand for the production of superior plants through genetic transformation. | |||||||||||||||||||||||||||||||||||||||||
| Learning Outcomes | |||||||||||||||||||||||||||||||||||||||||
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1. Compare and contrast the culture of animal cells, bacterial cells and viruses. 2. Discuss the problems associated with scaling up growth and describe methods for the measurement of growth in fermentations. 3. List a range of products formed during culture and discuss the factors involved in their formation. 4. Dissect experimental protocols from the scientific literature in plant micropropagation discussing thereasons for each step in the protocol 5. Dissect experimental protocols from the scientific literature in plant genetic transformation discussingthe reasons for each step in the protocol. | |||||||||||||||||||||||||||||||||||||||||
All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml |
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| Indicative Content and Learning Activities | |||||||||||||||||||||||||||||||||||||||||
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Animal, microbial and viral culture. Overview of Fermentation TechnologyMicrobial Cell CultureIntroduction and types of culture Ways of measuring growth Medium formulations Solid statefermentations Production formationAnimal Cell CultureIntroduction and types of animal cell culture Aspectic technique Growth media Growth factorsLarge scale animal cell culture Products produced by animal cell culture Genetic manipulationToxicity testing Cancer Biology. Starting Material. Sources of explants and the reasons for choosing them, and pre-conditioning prior to culture.. Sterility. Initial sterilisation of cultures and of media. Maintainance of sterility. Endemic bacteria and viruses - theirdetection and removal.. Plant Growth Regulators. Auxins; cytokinins; gibberellins; ethylene; abscisic acid. Non-conventional plant growth regulators.. Plant tissue culture media. Water; Salts; sugars; vitamins; undefined supplements.. Plant growth requirements. Light. Temperature and its control.. Regeneration. Organogenesis; 3 ways for somatic embyrogenesis. Micropropagation Cycle. Stage I - establishment of plant in culture Stage II - multiplication Stage III - hardening off Stage IV -transfer to soil.. Plant genetic transformation. The history of plant genetic transformation; Vectored V non-vectored transformation; Vector construction;Protocols for Agrobacterium-mediated transformation; Protocols for biolistic transformation; Ethical issues inplant genetic transformation;. | |||||||||||||||||||||||||||||||||||||||||
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| Indicative Reading List | |||||||||||||||||||||||||||||||||||||||||
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| Other Resources | |||||||||||||||||||||||||||||||||||||||||
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| Programme or List of Programmes | |||||||||||||||||||||||||||||||||||||||||
| BT | BSc in Biotechnology | ||||||||||||||||||||||||||||||||||||||||
| Timetable this semester: Timetable for BE316 | |||||||||||||||||||||||||||||||||||||||||
| Date of Last Revision | 14-APR-11 | ||||||||||||||||||||||||||||||||||||||||
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