Registry

Module Specifications

Current Academic Year 2012 - 2013
Please note that this information is subject to change.

Module Title	Protein Expression, Purification & Analysis
Module Code	BE383
School	School of Biotechnology
Online Module Resources
Module Co-ordinator	Semester 1: Brendan O'Connor Semester 2: Brendan O'Connor Autumn: Brendan O'Connor
Module Teacher	Brendan O'Connor
NFQ level	8	Credit Rating	5
Pre-requisite	None
Co-requisite	None
Compatibles	None
Incompatibles	None

Description

Module Aims:To provide a good practical 'hands-on' experience of a sequential multi-step protein purification scheme and affinity chromatography; To provide a practical experience of standard protein analytical methodologies ;To provide an understanding of the methods used to manipulate recombinant proteins ;To introduce the students to Fluorimetric analysis.

Learning Outcomes

1. Carry out a sequential 'multistep' protein purification scheme using salt precipitation, ion-exchange and gel filtration chromatographies.
2. Evaluate the effectiveness of a purification operation in terms of yield and purification factor.
3. Optimize the expression of a recombinant protein in a bacterial system.
4. Carry out the purification of atagged recombinant protein using affinity chromatography.
5. Perform polyacrylamide gel electrophoresis (PAGE) analysis of proteins with appropriate standards to assess purity using standard staining techniques.
6. Write up reports, to be viewed by an experimental scientist detailing their experimental findings and the conclusions that may be drawn from them.

Workload	Full-time hours per semester
*Type*	*Hours*	*Description*
Laboratory	48	No Description
Assignment	32	No Description
Directed learning	20	No Description
Independent learning	25	No Description
Total Workload: 125

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

Indicative Syllabus.

Assessment Breakdown
Continuous Assessment	100%	Examination Weight	0%

Course Work Breakdown
Type	Description	% of total	Assessment Date
Report (s) (written / oral)	n/a	100%	n/a

Reassessment Requirement
Resit arrangements are explained by the following categories; 1 = A resit is available for all components of the module 2 = No resit is available for 100% continuous assessment module 3 = No resit is available for the continuous assessment component
This module is category 2

Indicative Reading List

Other Resources

None

Array

Programme or List of Programmes

GCB

BSc in Genetics & Cell Biology

Timetable this semester: Timetable for BE383

Date of Last Revision

23-MAY-08

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