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Module Specifications

Current Academic Year 2012 - 2013
Please note that this information is subject to change.

Module Title Bioanalytical Practicals II
Module Code BE452
School School of Biotechnology
Online Module Resources

Module Co-ordinatorSemester 1: Dermot Walls
Semester 2: Dermot Walls
Autumn: Dermot Walls
NFQ level 8 Credit Rating 5
Pre-requisite None
Co-requisite None
Compatibles None
Incompatibles None
Description
This module builds on an understanding of concepts in molecular biology and immunology. It aims to teach a range of methods in these subject areas by providing training in selected relevant techniques.

Learning Outcomes
1. Isolate, quantitate and analyse nucleic acid preparations from various sources
2. Qualitatively analyse selected DNA sequences using methods including restriction endonuclease analysis, Polymerase Chain Reaction, and electrophoresis
3. Isolate and characterise polyclonal antibodies and conjugate antibodies with enzyme labels



Workload Full-time hours per semester
Type Hours Description
Laboratory48Eight six-hour laboratory sessions
Independent learning27Data interpretation and report writing
Directed learning50No Description
Total Workload: 125

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities
DNA from different sources will be mapped and differentiated by restriction endonuclease analysis followed by electrophoresis.
DNA from different sources will be differentiated by Polymerase Chain Reaction followed by electrophoresis.

Immunoglobulins will be isolated from animal serum, chemically conjugated to enzymes and used in enzyme linked immunosorbant assays.
Assessment Breakdown
Continuous Assessment100% Examination Weight0%
Course Work Breakdown
TypeDescription% of totalAssessment Date
Report (s) (written / oral)Data interpretation and report writing100%n/a
Reassessment Requirement
Resit arrangements are explained by the following categories;
1 = A resit is available for all components of the module
2 = No resit is available for 100% continuous assessment module
3 = No resit is available for the continuous assessment component
This module is category 2
Indicative Reading List
    Other Resources
    None
    Array
    Programme or List of Programmes
    ASBSc in Analytical Science
    Timetable this semester: Timetable for BE452
    Date of Last Revision26-MAY-08
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