Registry

Module Specifications

Current Academic Year 2012 - 2013
Please note that this information is subject to change.

Module Title	Bioseparations
Module Code	BE516
School	School of Biotechnology
Online Module Resources
Module Co-ordinator	Semester 1: Ian Marison Semester 2: Ian Marison Autumn: Ian Marison
Module Teacher	Ian Marison
NFQ level	9	Credit Rating	5
Pre-requisite	None
Co-requisite	None
Compatibles	None
Incompatibles	None

Description

The aim of this module is to provide a detailed understanding of the behaviour of proteins in solution and how their properties may be altered by changing the physical surroundings. To provide an understanding of the theory and practical techniques involved in developing a purification process.

Learning Outcomes

1. Discuss the principles of protein chemistry in solution.
2. Design a DSP process which is readily integrated with the USP
3. Decide how to choose the correct separation and chromatographic techniques.
4. Demonstrate an understanding of the theory and practice of chromatography and how this can help in designing the DSP.
5. Comment on the different types of chromatographic equipment and how they operate

Workload	Full-time hours per semester
*Type*	*Hours*	*Description*
Lecture	16	No Description
Independent learning	109	No Description
Total Workload: 125

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

Bioseparations.
This module is built on the fundamental concepts of protein structure and function, physical chemistry of solutions and electrolytes/ acids/bases and pH to understand how proteins behave in solution and how their properties may be altered by changing the physical surroundings.Using examples, descriptions will be given as to how to isolate proteins from inclusion bodies or culture medium using a range of chemical and physical separation processes.The principles of developing a purification process, including theory, will be defined using a wide range of chromatographic techniques including, but not exclusive to; gel filtration, ion-exchange, hydrophobic interaction, reverse phase, metal chelate, immunoaffinity and other affinity- based techniques, microfiltration and others.The different types of equipment, fluidized versus packed-beds and how these may be scaled-up will be discussed..

Assessment Breakdown
Continuous Assessment	100%	Examination Weight	0%

Course Work Breakdown
Type	Description	% of total	Assessment Date
Assignment	n/a	100%	n/a

Reassessment Requirement
Resit arrangements are explained by the following categories; 1 = A resit is available for all components of the module 2 = No resit is available for 100% continuous assessment module 3 = No resit is available for the continuous assessment component
This module is category 1

Indicative Reading List

Robert K.: 1994, Protein Purification – Principles & Practice, 3rd Edition, Scopes/Springer Verlag,
Paul Belter, E.L. Cussler, Wei-Shou Hu: 1988, Bioseperation Downstream Processing for Biotechnology, John Wiley & Sons,

Other Resources

None

Array

Programme or List of Programmes

GDBE

Graduate Dip in Bioprocess Engineering

MSBE

Master of Science in Bioprocess Engineer

SAMPB

STAND ALONE MODULE (BIOTECHNOLOGY)

SMPSC

Single Module Professional Science