Registry

Module Specifications

Current Academic Year 2012 - 2013
Please note that this information is subject to change.

Module Title	Introduction to Proteomics &Mass Spectrometry
Module Code	BE532
School	School of Biotechnology
Online Module Resources
Module Co-ordinator	Semester 1: Finbarr O'Sullivan Semester 2: Finbarr O'Sullivan Autumn: Finbarr O'Sullivan
Module Teacher	Finbarr O'Sullivan
NFQ level	8	Credit Rating	2.5
Pre-requisite	None
Co-requisite	None
Compatibles	None
Incompatibles	None

Description

The purpose of this module is to introduce participants to the technology currently available to analyse global patterns of gene expression at the protein level. The module will addresss applications of this technology to reveal gene regulation events involved in disease progression as well as potential targets for drug discovery and diagnostics.The module will cover the following aspects of proteomic technologies and research;• Sample preparation from various biological materials (e.g. cell lines, tissue, serum, plasma, etc.)• 2D Gel Electrophoresis, including 2D Difference Gel Electrophoresis (2D DIGE)• Mass Spectrometry (MS) for protein identification and characteristion• Quantitative LC-MS proteomic technologieso SILACo iTRAQo Label-free• Introduction to bioinformatics tools for proteomics

Learning Outcomes

1. Select the most appropriate method of sample preparation for various biological materials.
2. Discuss the advantages, limitations and challenges associated with analysis of the proteome by different techniques.
3. Demonstrate knowledge of the differences between the various mass spectrometry techniques for protein identification and characterization.
4. Have an ability to analyze and characterize a protein of interest using various protein bioinformatic software tools.

Workload	Full-time hours per semester
*Type*	*Hours*	*Description*
Lecture	12	No Description
Independent learning time	3	Reading
Examination	1	No Description
Total Workload: 16

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

None

Assessment Breakdown
Continuous Assessment	100%	Examination Weight	0%

Course Work Breakdown
Type	Description	% of total	Assessment Date
Multiple choice questions	n/a	100%	n/a

Reassessment Requirement
Resit arrangements are explained by the following categories; 1 = A resit is available for all components of the module 2 = No resit is available for 100% continuous assessment module 3 = No resit is available for the continuous assessment component
This module is category 1

Indicative Reading List

Other Resources

None

Array

Programme or List of Programmes

AMPD

PhD

AMPM

MSc

AMPT

PhD-track

BTPD