Spectroscopy and Chromatography

Thermo Scientific Nicolet RaptIR FTIR Microscope

The Nicolet RaptIR FTIR Microscope is a research-grade microscope coupled to a Nicolet FTIR spectrometer, designed to combine high-resolution imaging with infrared spectral analysis. It lets you locate features under visible light, then zoom in optically and chemically to characterise materials with >5 µm infrared spatial resolution and ~1 µm visible resolution. Whether using reflectance, transmission, or ATR modes, it supports diverse applications—including polymer studies, forensics, microplastics, environmental work, pharmaceuticals, and material contamination analysis.

Specifications

• High-performance visual (4X) and infrared (15X) imaging delivered by diffraction-limited objectives.
• Multi-position automated nosepiece, autofocus, auto illumination, and a full-view computer-controlled aperture.
• Visual spatial resolution at 1 micron, and infrared spatial resolution at better than 5 microns to obtain high-resolution images and absorbance spectra.
• Supports reflectance, transmission, and ATR sampling modes & includes built-in ATR sensors to deliver user-adjustable pressure control.
• Heavy-duty stage to accommodate samples up to 40 millimeters thick and 5 kilograms in weight.
• Fast results with mapping of up to 10 spectra/second.
• Automated data acquisition and analysis through OMNIC Paradigm software.
• Option to install a grazing angle infrared objective for thin-layer analysis. 

Applied Photophysics Chirascan Plus Circular Dichroism Spectroscopy

The Chirascan Plus CD Spectrometer is a versatile tool for probing the structure and stability of biomolecules and synthetic biomimetic polymers. It provides detailed information on secondary and tertiary structures, conformational changes, and molecular folding dynamics. Researchers can study protein and nucleic acid stability, ligand binding, and thermal unfolding, as well as monitor structural changes under varying environmental conditions such as temperature or solvent. Its flexibility and sensitivity make it an essential instrument for structural biology, biochemistry, and polymer research.

Specifications:

• Wavelength Range: 170–1,150 nm
• Wavelength Accuracy: ±0.2 nm (170–400 nm), ±0.5 nm (>400 nm)
• Wavelength Precision: ±0.05 nm (170–400 nm), ±0.1 nm (>400 nm)
• Resolution: 0.1 nm
• Stray Light: <5 ppm @ 200 nm, <8 ppm @ 180 nm
• Baseline Stability: ±0.02 mdeg/hr (@ 290 nm)
• RMS Noise: 0.02–0.05 mdeg (depending on wavelength)

Featured Publications:

Gibney, A. et al. (2023a) ‘A click chemistry‐based artificial metallo‐nuclease’, Angewandte Chemie International Edition, 62(38). doi:10.1002/anie.202305759.

Holden, L. et al. (2023b) ‘Selective, disruptive luminescent ru(ii) polypyridyl probes of G-Quadruplex’, Inorganic Chemistry, 62(5), pp. 2213–2227. doi:10.1021/acs.inorgchem.2c03903.

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Perkin Elmer LS55 Fluorescence Spectrometer

The PerkinElmer LS 55 Fluorescence Spectrometer is a versatile instrument for measuring fluorescence, phosphorescence, chemiluminescence, and bioluminescence in a wide range of samples. It enables detailed analysis of emission and excitation properties, helping researchers understand molecular environments, interactions, and dynamics. Typical applications include studying protein and nucleic acid fluorescence, monitoring enzyme activity, investigating chemical sensors, and characterizing materials with luminescent properties, making it a valuable tool in biochemistry, molecular biology, and materials science.

Specifications:

• Pulsed xenon source
• Excitation Range: 200–800 nm
• Emission Range: 200–900 nm
• Independent or synchronous scanning of monochromators to aid in method development and peak identification
• 4 measurement modes; Fluorescence, Phosphorescence, Chemiluminescence & Bioluminescence
• Excitation and emission scans
• Constant wavelength synchronous and constant energy synchronous scans
• 3D excitation/emission scans and 3D synchronous scans
• Kinetic scans (single and multiple wavelength)
• Simultaneous kinetics for multiple samples

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Shimadzu UV-2600 and UV-1800 UV-VIS Scanning Spectrophotometers 

The Shimadzu UV-2600 and UV-1800 UV-VIS Scanning Spectrophotometers are versatile instruments for the qualitative and quantitative analysis of compounds that absorb light in the ultraviolet and visible regions. They support a wide range of applications, including concentration determination, purity assessment, reaction monitoring, and optical property studies of materials. The UV-2600 offers extended measurement into the near-infrared (up to 1,400 nm with an integrating sphere), making it suitable for materials with broader absorption characteristics, while the UV-1800 provides reliable UV-VIS coverage up to 1,100 nm. Together, they provide flexible and precise measurement options for chemistry, life sciences, pharmaceuticals, and materials research.

Specifications

• Wavelength accuracy of ±0.3nm throughout the entire range
• Wavelength repeatability of ±0.05nm (2600) and ±0.1nm (1800)
• Resolution of ±0.1nm (2600) and  ±1nm (1800)
• Photometric range between -5 to +5 Abs (2600) and -4 to +4 Abs (1800)
• Photometric accuracy as low as ±0.002 Abs
• Photometric repeatability as low as ±0.001 Abs

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PerkinElmer Spectrum Two FTIR

The Spectrum Two is a versatile Fourier Transform Infrared (FTIR) spectrometer designed for rapid, reliable identification and analysis of a wide variety of materials. It provides detailed information on molecular structure and chemical composition, making it widely used for applications such as material verification, quality control, contamination analysis, and research in both academic and industrial settings. Its ease of use and broad applicability make it a practical tool for routine testing as well as more advanced investigations.

Specifications:

• Spectral range of 350 – 8,300 cm⁻¹
• High-performance DLATGS detector
• Better than 0.5 cm⁻¹ resolution
• Diamond Universal ATR (robust, versatile sampling option)

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Shimadzu Prominence HPLC

The Shimadzu Prominence HPLC system is a versatile high-performance liquid chromatography platform designed for separation, identification, and quantification of chemical compounds. It supports a wide range of applications including pharmaceutical analysis, biochemistry, environmental testing, and quality control. With precise solvent delivery, gradient control, and high reproducibility, the system allows researchers to perform complex analytical workflows with confidence.

Specifications:

• Pump flow range: 0.001 – 10 mL/min, precision ≤0.1% RSD
• Autosampler with injection volumes from 0.1 – 100 µL
• Detector options: UV-Vis & PDA
• Wavelength Range: 190 nm - 700 nm (+/-1 nm)
• Modular, high-sensitivity system for analytical and semi-preparative use
• Column oven: Columns < 150mm in length 25^oC - 150^oC, Columns < 310mm in length 25^oC - 90^oC

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