Name:

Dr. Yuliya Shakalisava

Contact details

Qualifications

PhD (2007) Dublin City University, Dublin, Ireland

Title: Further Investigation of Capillary Electrophoresis for Pharmaceutical Analysis

BSc (2002) Belarussian State University, Minsk, Belarus

Analitycal & Environmental Chemistry

Project Summary

The project aims for the development of an easy-to-use way of warning of possible faecal contamination of water. The microbiological parameters of water are of the particular concern in Ireland. The presence of bacteria, such as E. coli and enterococci, represent a significant danger to public health and is a sign of malfunctioning of water treatment plant or a supplementary contamination of water after treatment. Human and animal waste is the major sources of microbiological pollution in sewage and water environments. Monitoring of microbial water quality rely on culture growth methods and require at least 18-24 hours for analysis. This is not acceptable in the situation where immediate action has to be taken. The work is ongoing in order to evaluate several strategies for utilising surrogate measurements of water quality, such as turbidity, particle size and video sensing, as early warning systems for microbiological contamination, using parallel cell-based measurements for reference purpose. In the longer term, a microfluidic system is planned that can provide a more specific determination of E. coli contamination and will be capable of remote sampling & analysis over extended periods of time. This project is a part of a larger Beaufort Marine award, which involves a number of inter-related research projects. These range across biofouling issues, development of a miniaturised analytical device for marine monitoring, video and satellite sensing of environmental conditions, biorecognition for detection of marine contaminants and fluoroimmunoassay for bacterial sensing.

Publications

JOURNAL PUBLICATIONS:

1. M. Poitevin, Y. Shakalisava, G. Peltre, J-L Viovy, S. Descroix, Evaluation of microchip material and surface treatment options for isoelectric focusing of allergenic milk proteins on microchips, Electrophoresis, under revision, June 2009.

2. Y. Shakalisava and F. Regan, Capillary electrophoresis separation approaches for combination cancer drugs, Electrophoresis, accepted, May 2009.

3. Y. Shakalisava, M. Poitevin, J-L Viovy, S. Descroix, Versatile method for low EOF measurements in microchip CE. J. Chromatogr. A 1216 (6) (2009) 1030-1033.

4. Y. Shakalisava and F. Regan, Separation of montelukast sodium and related impurities by capillary electrophoresis. J. Separ. Sci. 31 (2008) 1137-1143.

5. Y. Shakalisava and F. Regan, Determination of association constants of inclusion complexes of steroid hormones and cyclodextrins from their electrophoretic mobility. Electrophoresis 27(15) (2006) 3048-3056.

6. Y. Shakalisava and F. Regan, Rapid simultaneous determination of alkylxanthines by CZE and its application in analysis of pharmaceuticals and food samples. Anal. Chim. Acta 540 (2005) 103-110.

ORAL PRESENTATIONS:

1. Biodetection Technologies 2009, Baltimore, MD, USA

“Early warning of microbiological contamination of water”

Yuliya Shakalisava

2. International Symposium on MicroScale Bioseparations 2008, Berlin, Germany

“Versatile method for low EOF measurements in microchip CE”

Yuliya Shakalisava

3. 58^th Irish Universities Chemistry Research Colloquium 2006, Galway, Ireland

 “A capillary electrophoretic study of the inclusion of steroid hormones in cyclodextrins” 

Yuliya Shakalisava

4. Merck Sharp & Dohme (Irl.) Ltd., company presentation 2005

“Development of capillary electrophoresis method for the determination of related impurities in montelukast sodium”

Yuliya Shakalisava

POSTER PRESENTATIONS:

1. MicroScale Bioseparations 2008, Berlin, Germany

 “Simultaneous determination anthracyclines and taxens by capillary electrophoresis”

Yuliya Shakalisava and Fiona Regan

2. ITP 2006, Paris, France

 “Determination of antiasthmatic compounds by capillary electrophoresis”

Yuliya Shakalisava and Fiona Regan

3. MicroScale Bioseparations 2006, Amsterdam, Netherlands

 “The potential of cyclodextrin in developing generic buffers for the separation of steroids”

Yuliya Shakalisava and Fiona Regan

4. MicroScale Bioseparations 2006, Amsterdam, Netherlands

 “Development of capillary electrophoresis method for the determination of related impurities in montelukast sodium”

Yuliya Shakalisava and Fiona Regan

5. MicroScale Bioseparations 2005, New Orleans, USA

 “Cyclodextrin-modified capillary electrophoresis determination of steroids”

Yuliya Shakalisava and Fiona Regan

6. Analytical Forum 2004, Warsaw, Poland

 “Capillary zone electrophoresis for the determination of alkylxanthines”

Yuliya Shakalisava and Fiona Regan

Further Information