Fluorescence Microscopy

Leica TCS SP8 Confocal & STED Super Resolution Microscope

The Leica TCS SP8 STED super-resolution microscope is based on the STED (STimulated Emission Depletion) principle developed by Nobel Prize laureate Stefan Hell.  The base of this system is a fluorescence confocal microscope which is suitable for use in top level biological and biomedical research and surface analysis in material science applications.

 

Image shows a large microscope with cell image on screen
  • The light sources include a pulsed 405 nm diode laser, a white light Laser which can provide excitation wavelengths from 470 nm - 670 nm, and a metal halide lamp for wide field imaging

  • 592 nm and 660 nm STED depletion lasers for super resolution imaging.

  • Fluorescent Lifetime Microscopy using HyD single molecule detectors

  • FLIM, FCS, FLCS, FRAP and FRET measurements.

  • 2 Internal standard PMT confocal detectors, 2 Internal HyD GaAsP SMD detectors for gated imaging and 1 CCD camera

  • 10x air, 20x, 40x and 63x (with motorized correction collar) water, 40x, 63x and 100x oil objectives.

  • Resonant scanner for imaging at 24 frames per second.

  • Galvoflow, SuperZ galvanometer

  • 37°C degree stage top CO² incubator for live cell imaging

  • Has both high resolution and flexibility to cover a wide range of experimental setups in fluorescent microscopy

Prices:  
Academic: €29/hr
Industry: On request

Request Access

Contact:  
Admin: research.facilities@dcu.ie
Technical: una.prendergast@dcu.ie

 


Nikon Eclipse Ti-E Fluorescence Microscope

The Nikon TiE is fully automated inverted widefield fluorescence microscope with builting Perfect Focus system and high-speed motorization. The Eclipse Ti-E inverted microscope offers improved system speed, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and with its environmental control, it is perfect for live cell imaging. It has a motorised and automated XYZ, Nikon’s Perfect Focus System and a 37°C degree stage top CO² incubator for live cell imaging

Widefield fluorescence image of cells stained with 3 dyes

Widefield fluorescence image of mammalian cells

 

Imaging Modes:

  • Multichannel (λ)

  • Multipoint (x,y,λ)

  • Z Stacks (x,y,z,λ)

  • Time Series (x,y,z,λ,t)

  • Large image stitching

Objectives:

  • 20x (air)

  • 40x (air)

  • 60x (oil)

Filter cubes:

  • DAPI

  • Alexa 488/ FITC/GFP

  • Cy3

  • Ruthenium

Prices:  
Academic: €12/hr
Industry: On request

 

Request Access

 

Contact:  
Admin: research.facilities@dcu.ie
Technical: una.prendergast@dcu.ie